{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:27:11Z","timestamp":1789698431842,"version":"4.0.1"},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000038","name":"U.S. Food and Drug Administration","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Human enucleated mature red blood cells (RBCs) contain both mature microRNAs (miRNAs) and mRNAs, and we have previously correlated RBC storage lesion processes such as eryptosis, adenosine 5\u2032\u2010triphosphate loss, and RBC indices with differentially expressed miRNAs. Here we have characterized Argonaute 2 (AGO2)\u2013miRNA complexes in stored mature RBCs as a first step toward understanding their role, if any.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>In this report AGO2\u2010bound miRNAs in mature RBCs isolated from RBCs collected from three different healthy donors and stored for 24 hours at 4 to 6\u00b0C were identified by anti\u2010AGO2 immunoprecipitation (IP) followed by next\u2010generation sequencing of the RNA isolated from the IP. The data were analyzed by various bioinformatics tools.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The analysis highlighted 28 mature AGO2\u2010bound miRNAs that are common to all three donors, representing 95.6% of the identified miRNAs. Among these, miR\u201016\u20105p (20.6%), miR\u2010451a\u20105p (16.7%), miR\u2010486\u20105p (12.6%), and miR\u201092a\u20103p (12.6%) are the most abundant miRNAs. Functional enrichment analysis for mRNA targets of the 28 common miRNAs identified molecules related to various diseases, biofunctions, and toxicity functions such as cardio\u2010, hepato\u2010, and nephrotoxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Overall, these results demonstrate the existence of multiple intracellular AGO2\u2010bound miRNAs in 24\u2010hour\u2010stored RBCs and warrant further experiments to determine whether AGO2\u2010miRNAs are functional in RBCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14325","type":"journal-article","created":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T03:02:50Z","timestamp":1505962970000},"page":"2995-3000","source":"Crossref","is-referenced-by-count":23,"title":["Analysis of Argonaute 2\u2013microRNA complexes in ex vivo stored red blood cells"],"prefix":"10.1111","volume":"57","author":[{"given":"Long","family":"Vu","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research, US Food and Drug Administration Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viswanath","family":"Ragupathy","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, US Food and Drug Administration Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, US Food and Drug Administration Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chintamani","family":"Atreya","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, US Food and Drug Administration Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002360"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02585.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2015.01.007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062039"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920210793175895"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-7-18"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12727"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13235"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2838"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv403"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120614"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1651"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-03-492801"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-461798"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.06.007"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13084"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14325","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T05:53:26Z","timestamp":1694757206000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,20]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/trf.14325"],"URL":"https:\/\/doi.org\/10.1111\/trf.14325","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,20]]}}}