{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:52:49Z","timestamp":1788450769363,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Isoantibodies against CD36 (platelet glycoprotein 4), developed in Type I CD36\u2010deficient mothers are frequently reported as the cause of fetal\/neonatal alloimmune thrombocytopenia in the Asian population. Therefore, further detailed characterization of anti\u2010CD36\u2013mediated fetal\/neonatal alloimmune thrombocytopenia is warranted. Here, we report the characterization of a patient with fetal\/neonatal alloimmune thrombocytopenia in a Taiwanese family caused by anti\u2010CD36 isoantibodies using a novel antigen\u2010capture method.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Platelets and monocytes were analyzed for CD36 expression by flow cytometry. Sequencing analysis of the CD36 gene was performed to identify the mutation underlying the CD36 deficiency. Stable transfected human embryonic kidney HEK293 cells expressing recombinant CD36 were established. These cells were used for the characterization of anti\u2010CD36 isoantibodies by flow cytometry, immunoprecipitation, and antigen\u2010capture assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Flow cytometry analysis revealed a total absence of CD36 on both platelets and monocytes of the mother (Type I CD36\u2010deficient) caused by heterozygous deletions of the CD36 gene (332_333delCA and c.1254\u2009+\u20096_1254\u2009+\u200911delTATTTG). Analysis of maternal serum with CD36\u2010transfected HEK293 cells by flow cytometry, immunoprecipitation, and antigen\u2010capture assay demonstrated the presence of anti\u2010CD36 isoantibodies in maternal serum. Interestingly, this antibody could not be detected by the monoclonal antibody immobilization of platelet antigens assay when anti\u2010CD36 monoclonal antibody (clone FA6\u2010152) was used as the capture antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This case reemphasizes the role of anti\u2010CD36 isoantibodies on the pathomechanism of fetal\/neonatal alloimmune thrombocytopenia. The fact that the monoclonal antibody immobilization of platelet antigens assay does not seem to be reliable for the identification of all anti\u2010CD36 antibodies indicates that screening of anti\u2010CD36 isoantibodies by a monoclonal antibody\u2010independent method, as presented here, should be considered.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14369","type":"journal-article","created":{"date-parts":[[2017,10,14]],"date-time":"2017-10-14T03:52:40Z","timestamp":1507953160000},"page":"189-195","source":"Crossref","is-referenced-by-count":21,"title":["Fetal\/neonatal alloimmune thrombocytopenia due to anti\u2010<scp>CD36<\/scp> antibodies: antibody evaluations by <scp>CD36<\/scp>\u2010transfected cell lines"],"prefix":"10.1111","volume":"58","author":[{"given":"Marie","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Medical Research Mackay Memorial Hospital Tamsui, New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzhang","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology and Transfusion Medicine, Justus\u2010Liebig University Giessen Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Lin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Research Mackay Memorial Hospital Tamsui, New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Der\u2010Cheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Mackay Memorial Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sentot","family":"Santoso","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology and Transfusion Medicine, Justus\u2010Liebig University Giessen Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.12.4402"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00645.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91733-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12235"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2004.00535.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.12.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.272re3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.2.392.392"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41030375.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00176.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2008.01093.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-05-0378"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH13-05-0435"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.6.1722.1722"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41121553.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1692"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/90.1.63"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20443"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1995.tb00369.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kjms.2013.07.005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12356"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V75.3.684.684"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12890"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.12.3545.3545"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21320"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1055\/s-0038-1653810","article-title":"A 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