{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:23:57Z","timestamp":1789025037525,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>In addition to titration by indirect antiglobulin test most widely used, anti\u2010D quantitation by continuous\u2010flow analysis (CFA) may be performed to assess severity of maternal immunization. Only five studies have reported its added value in the management of pregnancies complicated by anti\u2010D immunization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>A retrospective study of 74 severe anti\u2010D\u2013immunized pregnancies was conducted from January 1, 2013, to December 31, 2014, in the Trousseau Hospital in Paris (France). Concentration of maternal anti\u2010D was measured by titration and by CFA two\u2010stages method (2SM; total amount of anti\u2010D) and one\u2010stage method (1SM; high\u2010affinity IgG1 anti\u2010D). These biologic data were analyzed according to the severity of the hemolytic disease of the fetus and the newborn.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The value of 5 IU anti\u2010D\/mL in maternal serum is validated as a threshold to trigger ultrasonographic and Doppler fetal close follow\u2010up. A high 1SM\/2SM ratio was associated with a higher risk of intrauterine transfusion (IUT). For pregnancies requiring IUT and without increasing titer, maternal 1SM anti\u2010D concentration tends to correlate with the precocity of fetal anemia. In the \u201cwithout\u2010IUT\u201d group 1SM and 2SM anti\u2010D concentrations correlate significantly with cord bilirubin levels of the newborn at birth.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Altogether our results underline the importance of anti\u2010D quantitation by CFA to optimize the management of anti\u2010D\u2013alloimmunized pregnancies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.14406","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T12:45:49Z","timestamp":1511959549000},"page":"294-305","source":"Crossref","is-referenced-by-count":12,"title":["Clinical input of anti\u2010D quantitation by continuous\u2010flow analysis on autoanalyzer in the management of high\u2010titer anti\u2010D maternal alloimmunization"],"prefix":"10.1111","volume":"58","author":[{"given":"C\u00e9cile","family":"Toly\u2010Ndour","sequence":"first","affiliation":[{"name":"Unit\u00e9 Fonctionnelle d'expertise en Immuno\u2010H\u00e9mobiologie P\u00e9rinatale"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifa","family":"Mourtada","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Fonctionnelle Clinique (Soins des Incompatibilit\u00e9s Foeto\u2010maternelles et Ict\u00e8re N\u00e9onatal) Centre National de R\u00e9f\u00e9rence en H\u00e9mobiologie P\u00e9rinatale (CNRHP), Service de M\u00e9decine F\u0153tale, P\u00f4le P\u00e9rinatalit\u00e9, H\u00f4pital Trousseau, GH HUEP, APHP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Huguet\u2010Jacquot","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Fonctionnelle d'expertise en Immuno\u2010H\u00e9mobiologie 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N\u00e9onatal) Centre National de R\u00e9f\u00e9rence en H\u00e9mobiologie P\u00e9rinatale (CNRHP), Service de M\u00e9decine F\u0153tale, P\u00f4le P\u00e9rinatalit\u00e9, H\u00f4pital Trousseau, GH HUEP, APHP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Thomas","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Fonctionnelle Clinique (Soins des Incompatibilit\u00e9s Foeto\u2010maternelles et Ict\u00e8re N\u00e9onatal) Centre National de R\u00e9f\u00e9rence en H\u00e9mobiologie P\u00e9rinatale (CNRHP), Service de M\u00e9decine F\u0153tale, P\u00f4le P\u00e9rinatalit\u00e9, H\u00f4pital Trousseau, GH HUEP, APHP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Marie","family":"Jouannic","sequence":"additional","affiliation":[{"name":"D\u00e9partements de Gyn\u00e9cologie\u2010Obst\u00e9trique et de M\u00e9decine F\u0153tale, P\u00f4le P\u00e9rinatalit\u00e9 H\u00f4pital Trousseau, GH HUEP, 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