{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:16:49Z","timestamp":1787624209396,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T00:00:00Z","timestamp":1515542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T00:00:00Z","timestamp":1515542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Chikungunya virus, a mosquito\u2010borne arbovirus, often co\u2010circulates with the Zika, dengue, and yellow fever viruses in\n                      <jats:italic>Aedes<\/jats:italic>\n                      mosquito\u2010infested areas where cases of arbovirus transfusion\u2010transmitted infections have been reported. Building on past experience to help maintain the availability of safe components during major outbreaks of chikungunya virus in La Reunion, Italy, and Thailand and of Zika virus in the Pacific, the Caribbean, and the Americas, pathogen inactivation is a mitigation strategy to reduce the risk of transfusion\u2010transmitted infection. Inactivation of chikungunya virus was investigated for platelets in 100% plasma using amotosalen\/ultraviolet A light, and in red blood cells using amustaline\/glutathione.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>Platelets in 100% plasma and red blood cells (RBCs) were spiked with chikungunya virus. Infectious chikungunya virus titers were measured in contaminated blood products before and after treatment with amotosalen\/ultraviolet A light for platelets in 100% plasma and after treatment with amustaline\/glutathione for RBCs. Viral infectivity was quantified by plaque assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The mean chikungunya virus infectivity titers before inactivation were 6.50 log\n                      <jats:sub>10<\/jats:sub>\n                      plaque\u2010forming units\/mL for platelets in 100% plasma and 7.60 log\n                      <jats:sub>10<\/jats:sub>\n                      plaque\u2010forming units\/mL for RBCs. No infectivity was detected after amotosalen\/ultraviolet A light or amustaline\/glutathione treatment, corresponding to greater than 6.5 log\n                      <jats:sub>10<\/jats:sub>\n                      plaque\u2010forming units\/mL and greater than 7.1 log\n                      <jats:sub>10<\/jats:sub>\n                      plaque\u2010forming units\/mL of inactivation, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Robust levels of chikungunya virus inactivation were achieved for platelets in 100% plasma and for RBC components. The licensed amotosalen\/ultraviolet A light technology and the amustaline\/glutathione pathogen\u2010reduction system under development may provide an opportunity for comprehensive mitigation of the risk of chikungunya virus transfusion\u2010transmitted infection by plasma, platelets, and RBCs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.14442","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T22:56:54Z","timestamp":1515625014000},"page":"748-757","source":"Crossref","is-referenced-by-count":21,"title":["Inactivation of chikungunya virus in blood components treated with amotosalen\/ultraviolet\n                    <scp>A<\/scp>\n                    light or amustaline\/glutathione"],"prefix":"10.1111","volume":"58","author":[{"given":"Andrew","family":"Laughhunn","sequence":"first","affiliation":[{"name":"Cerus Corporation Concord California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Jang S.","family":"Huang","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute and Department of Diagnostic Medicine\/Pathobiolgoy Kansas State University Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana L.","family":"Vanlandingham","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute and Department of Diagnostic Medicine\/Pathobiolgoy Kansas State University Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion C.","family":"Lanteri","sequence":"additional","affiliation":[{"name":"Cerus Corporation Concord California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adonis","family":"Stassinopoulos","sequence":"additional","affiliation":[{"name":"Cerus Corporation Concord California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.1189"},{"key":"e_1_2_7_3_1","first-page":"765","article-title":"Neurological complications in chikungunya fever","volume":"55","author":"Rampal SM","year":"2007","journal-title":"J Assoc Physicians India"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60281-X"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08347"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2006.6.325"},{"key":"e_1_2_7_7_1","first-page":"1040","article-title":"Chikungunya fever diagnosed among international travelers\u2014United States, 2005\u20102006","volume":"55","author":"Centers for Disease Control and Prevention (CDC)","year":"2006","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1258\/td.2007.070019"},{"key":"e_1_2_7_9_1","first-page":"315","article-title":"Chikungunya epidemic: an Indian perspective","volume":"19","author":"Kalantri SP","year":"2006","journal-title":"Natl Med J India"},{"key":"e_1_2_7_10_1","first-page":"3030","article-title":"Imported cases of chikungunya in metropolitan France: update to June 2006","volume":"11","author":"Krastinova E","year":"2006","journal-title":"Euro Surveill"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61779-6"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1705.101873"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.28.20856"},{"key":"e_1_2_7_14_1","first-page":"1137","article-title":"Centers for Disease Control and Prevention. 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