{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:47:03Z","timestamp":1772988423663,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,17]],"date-time":"2017-12-17T00:00:00Z","timestamp":1513468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,12,17]],"date-time":"2017-12-17T00:00:00Z","timestamp":1513468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2016HH0062"],"award-info":[{"award-number":["2016HH0062"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700128"],"award-info":[{"award-number":["81700128"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Many emerging infectious pathogens are well known for existing in healthy blood donors and could be transmitted via blood transfusion or plasma derivatives usage. Therefore, there is an urgent need to discover the pathogens in qualified blood donation to avoid potential threats to blood safety.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>The objective of this study was to investigate the microbiome that existed in pooled plasma from different manufacturers in Chengdu and Guiyang. Random polymerase chain reaction, large\u2010scale clone sequencing, and bioinformatics were used to investigate the metagenomics and microbiome structure of pooled plasma. Among detected microbiomes, potential pathogens were subsequently identified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULT<\/jats:title>\n                    <jats:p>\n                      After host DNA cleaning, 551 clones were classified as bacteria; 88 clones were classified as viruses, and four clones were considered to be parasites, respectively. Thirteen kinds of bacteria and two kinds of parasites that might potentially threaten blood safety were identified along with six kinds of nonpathogenic viruses. The infection status of one identified pathogen\n                      <jats:italic>Coxiella burnetii<\/jats:italic>\n                      was evaluated in 1638 plasma samples. The reactive rate of immunoglobulin (Ig)G1 was 1.10% (18\/1638), the reactive rate of IgG2 was 0.85% (14\/1638), and the reactive rate of IgM was 0.98% (16\/1638).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Some pathogens that were already considered as threats to blood safety were discovered in those pooled plasma such as\n                      <jats:italic>C. burnetii<\/jats:italic>\n                      ,\n                      <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                      , and\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      sp. As a result, we should initiate some specific tests in the endemic area on plasma donors to enhance the blood safety in China.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.14455","type":"journal-article","created":{"date-parts":[[2017,12,17]],"date-time":"2017-12-17T23:16:51Z","timestamp":1513552611000},"page":"633-637","source":"Crossref","is-referenced-by-count":11,"title":["Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens"],"prefix":"10.1111","volume":"58","author":[{"given":"Min","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yashan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences and the Sichuan Blood Safety and Blood Substitute International Science and Technology Cooperation Base Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.7748\/en.23.9.31.s26"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tracli.2015.11.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1457496915622127"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13692"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853890009002022"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12096"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12070"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.10.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00454-09"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1993.33193142312.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02279.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03250.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-224"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38798346638.x"},{"key":"e_1_2_8_17_1","first-page":"595","article-title":"Escherichia coli O:H serotypes isolated from human blood. 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