{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:06:56Z","timestamp":1789625216648,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T00:00:00Z","timestamp":1519257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fresenius Kabi USA, LLC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Platelets (PLTs) collected and stored in PLT additive solution Intersol (PAS\u2010C) are presumed to reduce recipient exposure to donor plasma components; however, the effects of PAS\u2010C on PLT supernatant composition are poorly defined. Therefore, we compared the total protein concentration, isohemagglutinin titers, and HLA antibodies in supernatants of PAS\u2010C PLTs to plasma PLTs.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Apheresis PLTs from group O blood donors were collected into either 100% donor plasma (n\u00a0=\u00a050) or a mixture of 65% PAS\u2010C\/35% donor plasma (n\u00a0=\u00a050). Within 12 hours of collection, samples of the PLT supernatant were frozen and stored. PLT supernatants were assayed for total protein concentration and anti\u2010A and anti\u2010B titers and screened for HLA antibodies. Samples positive for HLA antibodies were further tested using single\u2010antigen bead assays to determine antibody strength and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Supernatants of PAS\u2010C PLTs had significantly lower total protein concentration and anti\u2010A and anti\u2010B titers compared to plasma PLTs. There was no significant difference in the number of HLA antibody screen\u2013positive PAS\u2010C (3\/50) compared to plasma PLT supernatants (2\/50); however, the HLA antibody screen\u2013positive supernatants of PAS\u2010C PLTs had fewer HLA specificities (2) compared to those of the plasma PLTs (18).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Decreased plasma proteins likely underlie lower rates of allergic and febrile, nonhemolytic transfusion reactions from the infusion of PAS\u2010C PLTs. Decreased anti\u2010A and anti\u2010B titers may prevent hemolysis from minor ABO mismatch. Lower HLA antibody specificities may mitigate transfusion\u2010related acute lung injury.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14523","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T06:26:18Z","timestamp":1519367178000},"page":"891-895","source":"Crossref","is-referenced-by-count":21,"title":["PAS\u2010C platelets contain less plasma protein, lower anti\u2010A and anti\u2010B titers, and decreased HLA antibody specificities compared to plasma platelets"],"prefix":"10.1111","volume":"58","author":[{"given":"Stuart P.","family":"Weisberg","sequence":"first","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth H.","family":"Shaz","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gizem","family":"Tumer","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris C.","family":"Silliman","sequence":"additional","affiliation":[{"name":"Department of Surgery Denver Health Medical Center Denver Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marguerite R.","family":"Kelher","sequence":"additional","affiliation":[{"name":"Department of Surgery Denver Health Medical Center Denver Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia S.","family":"Cohn","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12498"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12597"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12427"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06492.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13482"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02626.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-177857"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-10-532424"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e318186d87b"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12138"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.06.020"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1985.25686071434.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03048.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14523","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T06:38:59Z","timestamp":1695537539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,22]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/trf.14523"],"URL":"https:\/\/doi.org\/10.1111\/trf.14523","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,22]]}}}