{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:12:18Z","timestamp":1790255538834,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T00:00:00Z","timestamp":1518739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["142825\/2009\u20103"],"award-info":[{"award-number":["142825\/2009\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["475806\/2010\u20107"],"award-info":[{"award-number":["475806\/2010\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["7352\/12\u20103"],"award-info":[{"award-number":["7352\/12\u20103"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Neonatal alloimmune neutropenia results from maternal alloimmunization to human neutrophil antigens. The alloantibodies involved in neonatal alloimmune neutropenia are against human neutrophil antigens HNA\u20101a, HNA\u20101b, HNA\u20101c, HNA\u20101d, HNA\u20102, HNA\u20103a, HNA\u20104a, HNA\u20104b, and HNA\u20105a; however, to date, antibodies specific to HNA\u20103b have not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Blood samples from 10,000 unselected neonates were analyzed, resulting in the selection of 88 neutropenic newborns (neutrophil count &lt;1.5 \u00d7 10<jats:sup>9<\/jats:sup>\/L) from 83 mothers (three pairs of twins and one triplet). HNA\u20103 genotyping was performed by polymerase chain reaction\u2010restriction fragment length polymorphism to identify the cases of maternal\u2010fetal HNA\u20103 incompatibility. Serologic studies for detecting maternal HNA\u20103 alloantibodies were performed with the granulocyte agglutination test, the white blood cell immunofluorescence test, and a LABScreen Multi\u2010HNA Kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Genotyping studies identified 13 of 88 (14.8%) instances of maternal\u2010fetal HNA\u20103 incompatibility, with all mothers typed as HNA\u20103a\/a and neonates typed as HNA\u20103a\/b. Serologic studies revealed that five of 13 (38.5%) mothers carried anti\u2010HNA\u20103b plus human leukocyte antigen antibodies and that three of 13 (23.1%) mothers had anti\u2010HNA\u20103b without human leukocyte antigen antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Here, we report the first three cases of neonatal alloimmune neutropenia associated with HNA\u20103b antibodies resulting in a neonatal alloimmune neutropenia incidence of one in 3333 live births.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14524","type":"journal-article","created":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T11:46:07Z","timestamp":1518781567000},"page":"1264-1270","source":"Crossref","is-referenced-by-count":19,"title":["Antibodies to human neutrophil antigen HNA\u20103b implicated in cases of neonatal alloimmune neutropenia"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7837-166X","authenticated-orcid":false,"given":"Larissa Barbosa","family":"Lopes","sequence":"first","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira Ali","family":"Abbas","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elyse","family":"Moritz","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Oliveira","family":"Martins","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akemi Kuroda","family":"Chiba","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Dante M\u00e1rio","family":"Langhi","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 O.","family":"Bordin","sequence":"additional","affiliation":[{"name":"Disciplina de Hematologia e Hemoterapia da Universidade Federal de S\u00e3o Paulo, UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.1992.tb00148.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12086"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00199.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13287"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13463"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12326"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-248336"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2070"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12160"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13575"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03155.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12493"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37997454028.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02330.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12227"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02751.x"},{"key":"e_1_2_8_18_1","first-page":"236","article-title":"Prevalence of leukocyte alloantibodies in Brazilian multiparous blood donors","volume":"112","author":"Moritz E","year":"2017","journal-title":"Vox Sang"},{"key":"e_1_2_8_19_1","unstructured":"Instituto Brasileiro de Geografia e Estat\u00edstica (IBGE). 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