{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:53:51Z","timestamp":1789739631486,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T00:00:00Z","timestamp":1519257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Endothelial colony\u2010forming progenitor cells (ECFCs) are promising candidates for cell therapies. However, ECFC translation to the clinic requires optimized isolation and manufacture technologies according to good manufacturing practice (GMP).<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>ECFCs were manufactured from steady\u2010state peripheral blood (PB) leukapheresis (11 donors), using GMP\u2010compliant technologies including pooled human platelet (PLT) lysate, and compared to human umbilical cord endothelial cells, human aortic endothelial cells, and human cerebral microvascular endothelial cells. Specific variables assessed were growth kinetics, phenotype, trophic factors production, stimulation of tube formation, and Dil\u2010AcLDL uptake.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>ECFCs could be isolated from PB leukapheresis units with mean processed volume of 5411 mL and mean white blood cell (WBC) concentration factor of 8.74. The mean frequency was 1.44\u00a0\u00d7\u00a010<jats:sup>\u22128<\/jats:sup> ECFCs per WBC, corresponding to a mean of 177.8 ECFCs per apheresis unit. Expandable for up to 12 cumulative population doublings, calculated projection showed that approximately 730\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup> ECFCs could be manufactured from 1 apheresis unit. ECFCs produced epidermal growth factor, hepatocyte growth factor, vascular endothelial growth factor (VEGF)\u2010A, PLT\u2010derived growth factor\u2010B, interleukin\u20108, and monocyte chemoattractant protein\u20101, featured high potential for capillary\u2010like tubes formation, and showed no telomerase activity. They were characterized by CD29, CD31, CD44, CD105, CD117, CD133, CD144, CD146, and VEGF\u2010R2 expression, with the most common subpopulation CD34+CD117\u2013CD133\u2013. Compared to controls, ECFCs featured greater Dil\u2010AcLDL uptake and higher expression of CD29, CD31, CD34, CD44, CD144, and VEGF\u2010R2.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Here we show that isolation of ECFCs with proangiogenic profile from steady\u2010state PB leukapheresis is feasible, marking a first step toward ECFC product manufacture according to GMP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14541","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T06:26:27Z","timestamp":1519367187000},"page":"1132-1142","source":"Crossref","is-referenced-by-count":22,"title":["Manufacture of endothelial colony\u2010forming progenitor cells from steady\u2010state peripheral blood leukapheresis using pooled human platelet lysate"],"prefix":"10.1111","volume":"58","author":[{"given":"Georg","family":"Siegel","sequence":"first","affiliation":[{"name":"Institute for Clinical and Experimental Transfusion 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