{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:53:37Z","timestamp":1790322817193,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T00:00:00Z","timestamp":1520553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Reported hepatitis E virus (HEV) antibody assay performance characteristics are variable. Using a subset of surplus US blood donation samples, we compared assays for detecting anti\u2010HEV immunoglobulin M (Ig)M and IgG or total anti\u2010HEV antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Samples from 5040 random blood donations, all HEV\u2010RNA negative, collected primarily in the midwestern United States in 2015 were tested for anti\u2010HEV IgM and IgG or total anti\u2010HEV using assays manufactured by Diagnostic Systems, Wantai, and MP Biomedicals.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Overall, the percentage of detection for anti\u2010HEV IgG and total anti\u2010HEV was 11.4%, and for anti\u2010HEV IgM was 1.8%. Nine samples were reactive for anti\u2010HEV IgM by all assays, giving a recent infection rate of 0.18%. Anti\u2010HEV IgG\/total anti\u2010HEV detection rates increased with age. Interassay agreement was higher among the IgG anti\u2010HEV\/total anti\u2010HEV assays (84%) than the IgM assays (22%). Regression analyses of signal\u2010to\u2010cutoff ratios from IgG\/total antibody assay were heteroskedastic, indicating no constant variance among these assays, suggesting they may detect different epitopes or were affected by waning or less avid antibodies in the US donor population.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Although similar percentages of IgG anti\u2010HEV\/total anti\u2010HEV detection were observed across the three commercial assays, each assay detected a unique sample subpopulation and was heteroskedastic when compared pairwise. Discordance was higher among anti\u2010HEV IgM assays, but a recent HEV infection rate of at least 0.18% was estimated based on assay concordance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14553","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T05:26:44Z","timestamp":1520573204000},"page":"1254-1263","source":"Crossref","is-referenced-by-count":20,"title":["Disparities in detection of antibodies against hepatitis E virus in US blood donor samples using commercial assays"],"prefix":"10.1111","volume":"58","author":[{"given":"Mohammad","family":"Zafrullah","sequence":"first","affiliation":[{"name":"Division of Viral Hepatitis Centers for Disease Control and Prevention Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiugen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Viral Hepatitis Centers for Disease Control and Prevention Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coleen","family":"Tran","sequence":"additional","affiliation":[{"name":"Division of Viral Hepatitis Centers for Disease Control and Prevention Atlanta Georgia"},{"name":"MiMex Marietta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Nguyen","sequence":"additional","affiliation":[{"name":"American Red Cross Gaithersburg Maryland"},{"name":"US Food and Drug Administration Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleem","family":"Kamili","sequence":"additional","affiliation":[{"name":"Division of Viral Hepatitis Centers for Disease Control and Prevention Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2793-6522","authenticated-orcid":false,"given":"Michael A.","family":"Purdy","sequence":"additional","affiliation":[{"name":"Division of Viral Hepatitis Centers for Disease Control and Prevention Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Stramer","sequence":"additional","affiliation":[{"name":"American Red Cross Gaithersburg Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.02.007"},{"key":"e_1_2_8_3_1","unstructured":"PurdyMA SmithDB SimmondsP et al. New classification scheme for Hepeviridae ICTV taxonomic proposal 2014.008a\u2010hV.A.v6.Hepeviridae (approved). 2014 [cited 2018 Feb 20]. Available from:https:\/\/talk.ictvonline.org\/files\/ictv_official_taxonomy_updates_since_the_8th_report\/m\/vertebrate-official\/5150."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.068429-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.10.048"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811002925"},{"key":"e_1_2_8_7_1","first-page":"166","article-title":"Global epidemiology and medical aspects of hepatitis E","volume":"11","author":"Krawczynski K","year":"2001","journal-title":"Forum (Genova)"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/200824121023"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.06.016"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/655899"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/655898"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14025-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20364"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20834"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161025711"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ862"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.31.20550"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1712.110371"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12733"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu466"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12326"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13355"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit782"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit652"},{"key":"e_1_2_8_25_1","unstructured":"Centre National de Reference. Plan du rapport annuel d'activite: virus des hepatites a transmission enterique [Annual activity report plan: enteric transmission hepatitis virus; monograph on the Internet].2015. Available from:http:\/\/www.cnrvha-vhe.org\/wp-content\/uploads\/2012\/03\/2014-Rap-Act-VHE-VHA.pdf"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1086\/654801"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24741"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis688"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2307\/1911963"},{"key":"e_1_2_8_30_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2013"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25505"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Lozano R","year":"2010","journal-title":"Lancet"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1902.120961"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02343-15"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.12621"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2516(91)90067-D"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14307"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2016.1185362"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13498"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14553","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T13:53:45Z","timestamp":1695563625000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,9]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/trf.14553"],"URL":"https:\/\/doi.org\/10.1111\/trf.14553","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,9]]}}}