{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:43:05Z","timestamp":1792446185593,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:00:00Z","timestamp":1520899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The first step in the production of chimeric antigen receptor T cells is the collection of autologous T cells using apheresis technology. The procedure is technically challenging, because patients often have low leukocyte counts and are heavily pretreated with multiple lines of chemotherapy, marrow transplantation, and\/or radiotherapy. Here, we report our experience of collecting T lymphocytes for chimeric antigen receptor T\u2010cell manufacturing in pediatric and young adult patients with leukemia, non\u2010Hodgkin lymphoma, or neuroblastoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Apheresis procedures were performed on a COBE Spectra machine using the mononuclear cell program, with a collection target of 1 \u00d7 10<jats:sup>9<\/jats:sup> total mononuclear cells per kilogram. Data were collected regarding preapheresis and postapheresis blood counts, apheresis parameters, products, and adverse events.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Ninety\u2010nine patients (ages 1.3\u201025.7 years) and 102 apheresis events were available for analysis. Patients underwent apheresis at a variety of absolute lymphocyte cell counts, with a median absolute lymphocyte count of 944 cells\/\u03bcL (range, 142\u20106944 cells\/\u03bcL). Twenty\u2010two patients (21.6%) had absolute lymphocyte counts less than 500 cells\/\u03bcL. The mononuclear cell target was obtained in 100% of all apheresis harvests, and chimeric antigen receptor T\u2010cell production was possible from the majority of collections (94%). Mononuclear cell collection efficiency was 65.4%, and T\u2010lymphocyte collection efficiency was 83.4%. Ten patients (9.8%) presented with minor adverse events during the 102 apheresis procedures, with one exception of a severe allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Mononuclear cell apheresis for chimeric antigen receptor T\u2010cell therapy is well tolerated and safe, and it is possible to obtain an adequate quantity of CD3+ lymphocytes for chimeric antigen receptor T\u2010cell manufacturing in heavily pretreated patients who have low lymphocyte counts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14569","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T11:34:38Z","timestamp":1521113678000},"page":"1414-1420","source":"Crossref","is-referenced-by-count":89,"title":["Lymphocyte apheresis for chimeric antigen receptor T\u2010cell manufacturing in children and young adults with leukemia and neuroblastoma"],"prefix":"10.1111","volume":"58","author":[{"given":"Francesco","family":"Ceppi","sequence":"first","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Pediatric Hematology\u2010Oncology Research Laboratory &amp; Pediatric Hematology\u2010Oncology Unit, Division of Pediatrics, Department Woman\u2010Mother\u2010Child University Hospital of Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Rivers","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Department of Pediatrics University of Washington, Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen","family":"Annesley","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Department of Pediatrics University of Washington, Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navin","family":"Pinto","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Department of Pediatrics University of Washington, Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie R.","family":"Park","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Department of Pediatrics University of Washington, Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Lindgren","sequence":"additional","affiliation":[{"name":"Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Mgebroff","sequence":"additional","affiliation":[{"name":"Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Linn","sequence":"additional","affiliation":[{"name":"Apheresis Department Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan","family":"Delaney","sequence":"additional","affiliation":[{"name":"Children's National Health System  Washington, DC"},{"name":"Department of Pathology Seattle Children's Hospital Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca A.","family":"Gardner","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research, Seattle Children's Hospital and Research Institute Seattle Washington"},{"name":"Department of Pediatrics University of Washington, Bloodworks NW Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31405"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100369"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13792"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.018036"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61403-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1407222"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-02-769208"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300104"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1882"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PPO.0000000000000032"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI83416"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aad5222"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.03.001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2017.06.001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14094"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0354"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02443.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14569","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14569","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.14569","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14569","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T11:25:00Z","timestamp":1693221900000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14569"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,13]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1111\/trf.14569"],"URL":"https:\/\/doi.org\/10.1111\/trf.14569","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,13]]}}}