{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:36:34Z","timestamp":1761676594888,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Australian Red Cross Blood Service"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>D\u2013 individuals with previous D\u2010incompatible pregnancies and\/or blood transfusions, as well as those who are actively immunized with small\u2010volume D+ red blood cells (RBCs), are stimulated to produce RhIG. Many factors could influence the stimulation of immunoglobulin production in response to foreign antigen (such as antigen immunogenicity and genetic factors), and it is unknown whether genetic markers could potentially identify responder anti\u2010D donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Anti\u2010D donors were assigned a responder profile based on their serum RhIG levels (n\u2009=\u2009431). A subset of donors (n\u2009=\u2009272) had DNA extracted for polymerase chain reaction genotyping assays for target genes in antigen presentation and pathogen recognition receptors (TLR2, TLR4, CD14, Fc\u03b3RIIA, and the MHC Class II locus HLA\u2010DRB1). Statistical tests for associations between anti\u2010D donor responder profiles and genetic factors were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A large proportion of our donors (38.7%) were classified as nonresponder donors, despite receiving multiple D+ RBC immunizations, whereas female sex was significantly associated with an all\u2010responder profile (p\u2009&lt;\u20090.001). The presence of the DRB1*15 allele and absence of the DRB1*04 allele were more likely to be associated with a responder anti\u2010D donor, although not significantly after Bonferroni correction. A combination of the DRB1*15 allele and female sex was significantly associated with an anti\u2010D donor responder profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study has identified female sex and the HLA\u2010DRB1*15 allele as potentially useful markers that could be used to screen donors before entry into D immunization programs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14584","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T04:53:28Z","timestamp":1522126408000},"page":"1182-1188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["D\u2010immunized blood donors who are female and who possess at least one HLA\u2010DRB1*15 allele show a propensity for high serum RhIG production"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8700-6776","authenticated-orcid":false,"given":"Joanne C.G.","family":"Tan","sequence":"first","affiliation":[{"name":"Research and Development University of Sydney  Camperdown"},{"name":"Faculty of Medicine University of Sydney  Camperdown"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang Fang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Sydney  Camperdown"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackie","family":"Daley","sequence":"additional","affiliation":[{"name":"Manufacturing Australian Red Cross Blood Service Alexandria New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Marks","sequence":"additional","affiliation":[{"name":"Kirby Institute University of New South Wales Kensington New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Flower","sequence":"additional","affiliation":[{"name":"Research and Development University of Sydney  Camperdown"},{"name":"Faculty of Medicine University of Sydney  Camperdown"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne B.","family":"Dyer","sequence":"additional","affiliation":[{"name":"Research and Development University of Sydney  Camperdown"},{"name":"Faculty of Medicine University of Sydney  Camperdown"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2008.03735.x"},{"key":"e_1_2_8_3_1","first-page":"87","article-title":"Dynamics of the voluntary anti\u2010D plasma donor cohort in the Netherlands","volume":"105","author":"Berkowska MA","year":"2013","journal-title":"Vox 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