{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:00:18Z","timestamp":1783353618513,"version":"3.54.6"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T00:00:00Z","timestamp":1524528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Patient samples showing a positive indirect antiglobulin test are further tested to identify alloantibody specificity using a panel of phenotypically characterized group O reagent red blood cells (RBCs). Donor RBCs phenotypically negative for the antibody specificity are then serologically crossmatched using an antiglobulin reagent. This latter test is performed to identify any incompatibility due to the presence of undetected minor blood group antibodies and considered an important step in patient safety.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Samples with well\u2010characterized alloantibodies were intentionally crossmatched against donor RBCs expressing the cognate antigen. In a separate set of specimens, the alloantibody was titered and crossmatched against both heterozygous and homozygous cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Thirty\u2010five samples containing 10 common alloantibodies crossmatched against 240 ABO\u2010compatible donor cells phenotypically <jats:italic>positive<\/jats:italic> for the cognate antigen showed <jats:italic>compatible<\/jats:italic> crossmatches in 89 of 240 (37%). Antibody titering of 12 alloantibodies showed that a titer of 2 or more was required for incompatibility of all homozygous cells and a titer of 8 or more for incompatibility of all heterozygous cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The antiglobulin crossmatch has a high failure rate (false\u2010negatives) related to antibody titer and donor cell zygosity and is not reliable in interdicting incompatibility due to minor blood group antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14645","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T10:53:52Z","timestamp":1524567232000},"page":"2022-2026","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["False\u2010negative compatible antiglobulin crossmatches in samples with alloantibodies to cognate red blood cell antigens"],"prefix":"10.1111","volume":"58","author":[{"given":"Christian P.","family":"Nixon","sequence":"first","affiliation":[{"name":"Center for International Health Research Rhode Island Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie L.","family":"Krohto","sequence":"additional","affiliation":[{"name":"Blood Bank, The Miriam Hospital Providence Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph D.","family":"Sweeney","sequence":"additional","affiliation":[{"name":"Blood Bank, The Miriam Hospital Providence Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1981.21682085750.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1182\/blood.V59.5.873.873","article-title":"Pretransfusion tests and compatibility question of safety and efficacy","volume":"59","author":"Masouredis SP.","year":"1982","journal-title":"Blood"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(96)80088-5"},{"key":"e_1_2_7_5_1","first-page":"163","article-title":"The type and screen: a safe alternative and supplement in selected surgical procedures","volume":"17","author":"Henry JB","year":"1976","journal-title":"Transfusion"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1982.22282177114.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/82.2.178"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1986.26386209371.x"},{"key":"e_1_2_7_9_1","first-page":"412","article-title":"The risk of an overt hemolytic transfusion reaction following the use of an immediate spin crossmatch","volume":"114","author":"Shulman IA.","year":"1994","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb02995.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/94.4.428"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/86.4.511"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.5858\/2011-0127-CPR.1"},{"key":"e_1_2_7_14_1","volume-title":"Standards for blood banks and transfusion services","year":"2013"},{"key":"e_1_2_7_15_1","unstructured":"Transfusion medicine checklist. 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