{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:00Z","timestamp":1792787100195,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,16]],"date-time":"2018-05-16T00:00:00Z","timestamp":1526428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Taiwan Blood Services Foundation Research Program","award":["PM-105-TP-158"],"award-info":[{"award-number":["PM-105-TP-158"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      ABO subtypes are characterized by the alteration of antigens present and their expression levels on red blood cells and many are linked to genetic changes in the\n                      <jats:italic>ABO<\/jats:italic>\n                      gene. Weakened expression of antigens should be identified to prevent transfusion reactions or ABO\u2010incompatible transplantations. Genotyping can be applied to identify subtypes to complement serologic testing. Next\u2010generation sequencing (NGS) has shown to provide sensitive and accurate genotyping results as well as valuable cis\/trans information. Here we took advantage of NGS and applied it to resolve serology discrepancies in ABO typing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>\n                      In this study, we customized capture probes targeting the entire\n                      <jats:italic>ABO<\/jats:italic>\n                      gene and sequenced on MiSeq Illumina. The subtype\u2010causing variants were identified, and cis\/trans association to\n                      <jats:italic>ABO<\/jats:italic>\n                      alleles was determined. The results from NGS, serology, and Sanger sequencing were compared.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Four control samples typed A, B, O, and AB were correctly genotyped. Of 24 serologically discrepant samples, subtype\u2010causing variations were found in 20 cases, with two unresolved and two identified as weakening of ABO antibody in reverse. The types of variations include 17 known subtype alleles, one novel variant, one novel large deletion, and one microchimerism. Haplotypes encompassing Exons 6 and 7 of\n                      <jats:italic>ABO<\/jats:italic>\n                      were reconstructed in 17 of the 20 samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study demonstrated a full coverage of\n                      <jats:italic>ABO<\/jats:italic>\n                      by capture\u2010based panel, phasing analysis with NGS in\n                      <jats:italic>ABO<\/jats:italic>\n                      genotyping resolved heterogeneity with novel allele and microchimerism findings. This approach provided a more precise method for subtyping and thereby leading to safer transfusion.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.14654","type":"journal-article","created":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T00:40:08Z","timestamp":1526517608000},"page":"2232-2242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["ABO genotyping with next\u2010generation sequencing to resolve heterogeneity in donors with serology discrepancies"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2756-2479","authenticated-orcid":false,"given":"Ping Chun","family":"Wu","sequence":"first","affiliation":[{"name":"Taipei Blood Center, Taiwan Blood Services Foundation National Taiwan University Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin\u2010Hung","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Medical Genomics and Proteomics National Taiwan University Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei Fang","family":"Tsai","sequence":"additional","affiliation":[{"name":"Taipei Blood Center, Taiwan Blood Services Foundation National Taiwan University Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming Hung","family":"Chen","sequence":"additional","affiliation":[{"name":"Taipei Blood Center, Taiwan Blood Services Foundation National Taiwan University Hospital Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Lung","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Medical Genomics and Proteomics National Taiwan University Hospital Taipei 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