{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:14:56Z","timestamp":1789877696093,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T00:00:00Z","timestamp":1537142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondo de Investigacion Sanitaria Instituto de Salud Carlos III","award":["Red RETICEF RD06\/00130001"],"award-info":[{"award-number":["Red RETICEF RD06\/00130001"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The development of new noninvasive approaches for the diagnosis of human platelet antigen (HPA)\u20101 fetomaternal incompatibility has become of great interest. These approaches allow determination of whether the fetus is incompatible or not with the mother and a decision on antenatal therapy to avoid fetal or neonatal alloimmune thrombocytopenia (FNAIT). The objective of this work was to perform rapid, noninvasive prenatal test for HPA\u20101ab fetal antigen detection after the detection of an HPA\u20101\u2013homozygous mother by using plasma cell\u2010free DNA (cfDNA).<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>The HPA\u20101 genotypes of 142 pregnant women and 17 nonpregnant controls were retrospectively determined by high\u2010resolution melting (HRM) polymerase chain reaction (PCR). Coamplification at lower denaturation temperature (COLD) HRM PCR was performed to determine the fetal genotype analyzing cfDNA from all HPA\u20101bb pregnant women.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>After the HRM analysis, the following genotypes were identified: HPA\u20101aa (71.13%), HPA\u20101bb (2.8%), and HPA\u20101ab (26.06%). Four HPA\u20101bb\u2013homozygous pregnant women were carrying an incompatible fetus. Plasma samples from these mothers were analyzed by HRM COLD\u2010PCR. Homozygous HPA\u20101bb pregnant women carrying an HPA\u20101ab\u2013heterozygous fetus did not group with either the HPA\u20101ab or the HPA\u20101bb controls. Thus, COLD\u2010PCR analysis allows the detection of HPA\u20101ab\u2013heterozygous fetuses carried by homozygous mothers during first weeks of pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The fetal genotype from HPA\u20101bb\u2013homozygous women was detected by a noninvasive prenatal test as soon as 12 weeks of gestation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14837","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T17:58:19Z","timestamp":1537207099000},"page":"2272-2279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Noninvasive prenatal diagnosis by cell\u2010free DNA screening for fetomaternal HPA\u20101a platelet incompatibility"],"prefix":"10.1111","volume":"58","author":[{"given":"Marta","family":"Ferro","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas de Sevilla, IBIS (Universidad de Sevilla, HUVR, Junta de Andaluc\u00eda, CSIC) Hospital Universitario Virgen del Roc\u00edo\u2010Universidad de Sevilla  Seville Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hada C.","family":"Macher","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas de Sevilla, IBIS (Universidad de Sevilla, HUVR, Junta de Andaluc\u00eda, CSIC) Hospital Universitario Virgen del Roc\u00edo\u2010Universidad de Sevilla  Seville Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Forn\u00e9s","sequence":"additional","affiliation":[{"name":"the Blood Transfusion Center and Tissue and Cells Establishment C\u00f3rdoba  Cordoba Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Mart\u00edn\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"the Departmento de Hematolog\u00eda Hospital Universitario Virgen del Roc\u00edo  Seville Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Jimenez\u2010Arriscado","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas de Sevilla, IBIS (Universidad de Sevilla, HUVR, Junta de Andaluc\u00eda, CSIC) Hospital Universitario Virgen del Roc\u00edo\u2010Universidad de Sevilla  Seville Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrocinio","family":"Molinero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas de Sevilla, IBIS (Universidad de Sevilla, HUVR, Junta de Andaluc\u00eda, CSIC) Hospital Universitario Virgen del Roc\u00edo\u2010Universidad de Sevilla  Seville Andaluc\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"P\u00e9rez\u2010Sim\u00f3n","sequence":"additional","affiliation":[{"name":"the Departmento de Hematolog\u00eda 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