{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:44:08Z","timestamp":1788396248293,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Collagen\u2010 and thrombin\u2010activated (COAT) platelets (PLTs), generated by dual\u2010agonist stimulation with collagen and thrombin (THR), enhance THR generation at the site of vessel wall injury. There is evidence that higher amounts of procoagulant COAT PLTs are associated with stroke, while a decreased ability to generate them is associated with bleeding diathesis. Our aim was to study PLT functions, particularly the ability to generate COAT PLTs, in PLT concentrates (PCs) from buffy coat. Thus, we investigated the effect of processing, pathogen inactivation treatment (amotosalen\u2010UVA), and PC storage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>Two PCs from five donors each were pooled and split in two bags; one of them was pathogen inactivated and the other one was left untreated (n = 5). Flow cytometric analyses were performed immediately after PC preparation (Day 1) and thereafter on Days 2, 5, 7, and 9 in treated and untreated PCs to measure the reactivity of PLTs (CD62P and PAC\u20101), the content and secretion of dense granule after stimulation with different agonists, and the percentage of COAT PLTs after dual stimulation with convulxin (agonist of the collagen receptor GPVI) and THR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Preparation of PCs resulted in a significant decrease of COAT PLTs and in an impaired response to adenosine 5\u2032\u2010diphosphate sodium (ADP). Storage further decreased ADP response. Minor differences were observed between untreated or amotosalen\u2010UVA\u2013treated PCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Preparation of PCs from buffy coats decreased the ability to generate COAT PLTs and impaired PLT response to ADP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.14883","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T06:07:05Z","timestamp":1537337225000},"page":"2395-2406","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Generation of procoagulant collagen\u2010 and thrombin\u2010activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9756-4974","authenticated-orcid":false,"given":"Debora","family":"Bertaggia Calderara","sequence":"first","affiliation":[{"name":"Division of Hematology and Central Hematology Laboratory CHUV, Lausanne University Hospital and University of Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Crettaz","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche sur les Produits Sanguins Transfusion Interr\u00e9gionale CRS Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9382-138X","authenticated-orcid":false,"given":"Alessandro","family":"Aliotta","sequence":"additional","affiliation":[{"name":"Division of Hematology and Central Hematology Laboratory CHUV, Lausanne University Hospital and University of Lausanne Lausanne Switzerland"},{"name":"Facult\u00e9 de Biologie et de M\u00e9decine Universit\u00e9 de Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Barelli","sequence":"additional","affiliation":[{"name":"Division of Hematology and Central Hematology Laboratory CHUV, Lausanne University Hospital and University of Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Daniel","family":"Tissot","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche sur les Produits Sanguins Transfusion Interr\u00e9gionale CRS Epalinges Switzerland"},{"name":"Facult\u00e9 de Biologie et de M\u00e9decine Universit\u00e9 de Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-0179","authenticated-orcid":false,"given":"Michel","family":"Prudent","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche sur les Produits Sanguins Transfusion Interr\u00e9gionale CRS Epalinges Switzerland"},{"name":"Facult\u00e9 de Biologie et de M\u00e9decine Universit\u00e9 de Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9686-9920","authenticated-orcid":false,"given":"Lorenzo","family":"Alberio","sequence":"additional","affiliation":[{"name":"Division of Hematology and Central Hematology Laboratory CHUV, Lausanne University Hospital and University of Lausanne Lausanne Switzerland"},{"name":"Facult\u00e9 de Biologie et de M\u00e9decine Universit\u00e9 de Lausanne Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2012.01662.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2014.12.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08635.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0932"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08227.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02694.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2017.5123"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13123"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2014.02.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13638"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000403245"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-013-0813-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12173"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18020387"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2017.4949"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2010.02.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2009.07.002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-016-1136-0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12834"},{"key":"e_1_2_7_21_1","first-page":"255","article-title":"In vitro evaluation of pathogen\u2010inactivated buffy coat\u2010derived platelet concentrates during storage: psoralen\u2010based photochemical treatment step\u2010by\u2010step","volume":"13","author":"Abonnenc M","year":"2015","journal-title":"Blood Transfus"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000264666"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14310"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13690"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.5.1694.005k24_1694_1702"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/415175a"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH16-09-0711"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01274.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21157"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.301790"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-07-037283"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.168"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12045"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01430.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03179.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb03315.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12710"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614049"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2893"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00837.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00702.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00144.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1160\/TH04-09-0597","article-title":"Platelet P2Y12 receptors enhance signalling towards procoagulant activity and thrombin generation. A study with healthy subjects and patients at thrombotic risk","volume":"93","author":"Meijden PE","year":"2005","journal-title":"Thromb Haemost"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2008.03052.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2004.00628.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40101257.x"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40111320.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00349.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00923.x"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02092.x"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12364"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03811.x"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb08280.x"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-151043"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01167.x"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.7.2469"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.164137"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00017"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13000"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12317"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2007.05.004"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14883","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14883","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T07:46:56Z","timestamp":1694850416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,19]]},"references-count":60,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/trf.14883"],"URL":"https:\/\/doi.org\/10.1111\/trf.14883","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,19]]},"assertion":[{"value":"2018-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}