{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T16:23:54Z","timestamp":1768321434316,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Sterility testing of peripheral blood stem cells (PBSCs) is mandatory before release. As antibiotic treatment of the PBSC donor may result in false\u2010negative results, PBSC matrix validation must be carried out.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Three spiked PBSCs and a buffy coat (BC; control matrix) were analyzed using the blood culture device BacT\/ALERT 3D with the low\u2010temperature module. Samples were spiked with <jats:italic>Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, <jats:styled-content style=\"fixed-case\">Candida albicans<\/jats:styled-content>, Aspergillus brasiliensis, Clostridium sporogenes,<\/jats:italic> and <jats:italic>Propionibacterium acnes<\/jats:italic>. Standard iAST\/iNST culture bottles and iFA\/iFN Plus bottles, which include resorbing polymers, were incubated for 14 days. All aerobic bottles were incubated at 22.5\u00b0C and for a direct comparison also at 35\u00b0C while all anaerobic bottles were incubated at 35\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The BacT\/ALERT 3D system detected all microbes in iAST\/iNST culture bottles according to their growth behavior in the BC matrix. Detection of microbes differed significantly in PBSC products using standard iAST\/iNST culture bottles and iFA\/iFN Plus bottles with resorbing polymers: In Graft 1 no growth was detected in spiked bottles with <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> (iAST), <jats:italic>B. subtilis<\/jats:italic> (iAST\/iNST), <jats:italic>C. sporogenes<\/jats:italic> (iNST), and <jats:italic>P. acnes<\/jats:italic> (iNST) compared to iFA Plus and iFN Plus bottles wherein growth of spiked microbes was confirmed. Graft 2, with another antibiotic treatment, showed no growth in iAST\/iNST bottles spiked with <jats:italic>P. aeruginosa, B. subtilis,<\/jats:italic> and <jats:italic>C. sporogenes<\/jats:italic>. However, using iFA\/iFN Plus bottles all spiked microbes were detectable. The comparison of incubation temperature showed an expected slower growth at 22.5\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The use of iFA\/iFN Plus culture bottles incubated at different temperatures safely detected microbes in spiked PBSCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14910","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T14:40:36Z","timestamp":1538059236000},"page":"2918-2923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sterility release testing of peripheral blood stem cells for transplantation: impact of culture bottles and incubation temperature"],"prefix":"10.1111","volume":"58","author":[{"given":"Nicole","family":"Arlt","sequence":"first","affiliation":[{"name":"German Red Cross Blood Donor Service North\u2010East, Institute Cottbus  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remo","family":"Rothe","sequence":"additional","affiliation":[{"name":"German Red Cross Blood Donor Service North\u2010East, Institute Cottbus  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susann","family":"Sielaff","sequence":"additional","affiliation":[{"name":"German Red Cross Blood Donor Service North\u2010East, Institute Cottbus  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Juretzek","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene Carl\u2010Thiem Clinic  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidrun","family":"Peltroche","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene Carl\u2010Thiem Clinic  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-697X","authenticated-orcid":false,"given":"Rainer","family":"Moog","sequence":"additional","affiliation":[{"name":"German Red Cross Blood Donor Service North\u2010East, Institute Cottbus  Cottbus Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,27]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"963","article-title":"Microbial safety of cell based medicinal products\u2014what can we learn from cellular blood components?","author":"Montag T","year":"2008","journal-title":"Clin Chem Lab Med"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12097"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1994.tb05034.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1996.d01-57.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36996420754.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36996420753.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00013.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00573.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240310003044"},{"key":"e_1_2_7_11_1","first-page":"1232","article-title":"Quality control of bacterial contamination in autologous peripheral blood stem cells for transplantation","volume":"89","author":"Larrea L","year":"2004","journal-title":"Haematologica"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04178.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01165.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705731"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03359.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2013.04.026"},{"key":"e_1_2_7_17_1","first-page":"87","article-title":"Microbial contamination of harvested bone marrow and peripheral blood","volume":"15","author":"Prince HM","year":"1995","journal-title":"Bone Marrow Transplant"},{"key":"e_1_2_7_18_1","first-page":"1","article-title":"Richtlinie zur Herstellung und Anwendung von h\u00e4matopoetischen Stammzellzubereitungen","volume":"111","year":"2014","journal-title":"Dtsch \u00c4rztebl"},{"key":"e_1_2_7_19_1","unstructured":"Paul\u2010Ehrlich\u2010Institut\u2010Microbial Safety. 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