{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:25:07Z","timestamp":1780449907805,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The cost\u2010benefit question of general screening of blood products for the hepatitis E virus (HEV) is currently being discussed. One central question is the need for individual nucleic acid amplification techniques (NAT) screening (ID\u2010NAT) versus minipool NAT screening (MP\u2010NAT) approaches to identify all relevant viremias in blood donors. Here, the findings of ID\u2010NAT versus MP\u2010NAT in pools of 96 samples were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>From November 2017 to January 2018, a total of 10,141 allogenic blood donations from 7650 individual German blood donors were screened for the presence of HEV RNA using MP\u2010NAT (96 samples) (RealStar HEV RT\u2010PCR Kit) compared to ID\u2010NAT (cobas HEV assay) on the fully automated cobas 6800 platform.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Parallel screening of MP (n = 122, 96 samples\/MP) using both methods detected seven reactive pools. After pool resolution, 8 HEV RNA\u2013positive donations were identified by the in\u2010house detection method, whereas 17 HEV RNA\u2013positive donations were identified by ID\u2010NAT with the cobas HEV assay. This resulted in an incidence of 1:1268 donations (0.079%) for MP\u2010NAT screening and 1:597 donations (0.168%) for ID\u2010NAT screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The detection frequency of HEV RNA was approximately 50% higher if ID\u2010NAT was used compared to MP\u2010NAT. However, viral loads of ID\u2010NAT\u2013only samples were below 25 IU\/mL and will often not result in transfusion\u2010transmitted HEV (TT\u2010HEV) infection, taking into account the currently known infectious dose of 5.0E + 04 IU inevitably resulting in TT\u2010HEV infection. The clinical relevance and need for identification of these low\u2010level HEV\u2010positive donors still require further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15058","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T13:22:47Z","timestamp":1544707367000},"page":"612-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Hepatitis E virus blood donor NAT screening: as much as possible or as much as needed?"],"prefix":"10.1111","volume":"59","author":[{"given":"T.","family":"Vollmer","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Laboratoriums\u2010 und Transfusionsmedizin, Herz\u2010 und Diabeteszentrum Nordrhein\u2010 Westfalen, Universit\u00e4tsklinik der Ruhr\u2010Universit\u00e4t Bochum  Bad Oeynhausen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Diekmann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Laboratoriums\u2010 und Transfusionsmedizin, Herz\u2010 und Diabeteszentrum Nordrhein\u2010 Westfalen, Universit\u00e4tsklinik der Ruhr\u2010Universit\u00e4t Bochum  Bad Oeynhausen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Knabbe","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Laboratoriums\u2010 und Transfusionsmedizin, Herz\u2010 und Diabeteszentrum Nordrhein\u2010 Westfalen, Universit\u00e4tsklinik der Ruhr\u2010Universit\u00e4t Bochum  Bad Oeynhausen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dreier","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Laboratoriums\u2010 und Transfusionsmedizin, Herz\u2010 und Diabeteszentrum Nordrhein\u2010 Westfalen, Universit\u00e4tsklinik der Ruhr\u2010Universit\u00e4t Bochum  Bad Oeynhausen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000357098"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7920072.x"},{"key":"e_1_2_8_4_1","unstructured":"Human plasma (pooled and treated for virus inactivation) monograph 1646. 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