{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:43:19Z","timestamp":1767915799633,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T00:00:00Z","timestamp":1543449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sanquin","award":["PPOD-14-16"],"award-info":[{"award-number":["PPOD-14-16"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Blood\u2010group typing of donors and patients is essential to avoid incompatible transfusions. Transfusion of incompatible RBCs may result in alloimmunization complicating future transfusions or in the presence of antibodies in adverse reactions. With more than 300 blood group antigens identified, it is difficult to provide fully compatible blood. Currently, standard practice is to match for the most immunogenic antigens. While the current agglutination\u2010based RBC\u2010typing methods are reliable for testing a selected number of antigens, they are not easily adaptable for high\u2010throughput multiplex blood typing beyond the current standard.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Surface plasmon resonance (SPR) is a label\u2010free method to follow molecular\u2014and, very recently, also cellular\u2014interactions in real time. Demonstration of binding of RBCs to blood group antigen\u2010specific antibodies by SPR has already been achieved. Here, we demonstrate the generation of an SPR array equipped with clinically relevant blood group antibodies (A, B, and Rh blood groups). To validate this method, we blindly compared typing of 946 blood donors with results of current diagnostic agglutination\u2010based methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>RBC typing was achieved by monitoring RBC binding to blood group\u2013specific antibodies on the sensor simultaneously within 5 minutes per sample. Regeneration of the chip was robust, allowing for typing of at least 100 samples. The typing results gave a 100% match with classical serology with all antibodies tested besides anti\u2010E\/e monoclonals, which gave inconsistent results due to low antibody specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study demonstrates that SPR\u2010based RBC typing for multiple antigens can be realized simultaneously with high\u2010quality antibodies, enabling reduced hands\u2010on time and possibly improving cost efficiency.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15071","type":"journal-article","created":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T13:40:56Z","timestamp":1543498856000},"page":"754-761","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Multiplex blood group typing by cellular surface plasmon resonance imaging"],"prefix":"10.1111","volume":"59","author":[{"given":"Zoltan","family":"Szittner","sequence":"first","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur E.H.","family":"Bentlage","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"van der Donk","sequence":"additional","affiliation":[{"name":"Department of Reagents Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Ligthart","sequence":"additional","affiliation":[{"name":"Department of Diagnostics, Sanquin Research and Landsteiner Laboratory Academic Medical Centre, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Lissenberg\u2010Thunnissen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Ellen","family":"van der Schoot","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,29]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"ISBT Working Party on Red Cell Immunogenetics and Blood Group Terminology. Currently recognized blood group antigens. 2017 [cited 2017 Aug 29]. 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