{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T07:47:19Z","timestamp":1747986439549},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T00:00:00Z","timestamp":1548892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009425","name":"Landsteiner Foundation for Blood Transfusion Research","doi-asserted-by":"publisher","award":["1351"],"award-info":[{"award-number":["1351"]}],"id":[{"id":"10.13039\/100009425","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Alloimmunization against the high\u2010frequency Vel blood group antigen may result in transfusion reactions or hemolytic disease of fetus and newborn. Patients with anti\u2010Vel alloantibodies require Vel\u2010negative blood but Vel\u2010negative individuals are rare (1:4000). Identification of Vel\u2010negative donors ensures availability of Vel\u2010negative blood; however, accurate Vel blood group typing is difficult due to variable Vel antigen expression and limited availability of anti\u2010Vel typing sera. We report the production of a recombinant anti\u2010Vel that also identifies weak Vel expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>A recombinant anti\u2010Vel monoclonal antibody was produced by cloning the variable regions from an anti\u2010Vel\u2013specific B cell isolated from an alloimmunized patient into a vector harboring the constant regions of immunoglobulin (Ig)G1\u2010kappa or IgM\u2010kappa. Antibody Vel specificity was tested by reactivity to SMIM1\u2010transfected HEK293T cells and by testing various red blood cells (RBCs) of donors with normal, weak, or no Vel expression. High\u2010throughput donor screening applicability was tested using an automated blood group analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A Vel\u2010specific IgM class antibody was produced. The antibody was able to distinguish between Vel\u2010negative and very weak Vel antigen\u2013expressing RBCs by direct agglutination and in high\u2010throughput settings using a fully automated blood group analyzer and performed better than currently used human anti\u2010Vel sera. High\u2010throughput screening of 13,288 blood donations identified three new Vel\u2010negative donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We generated a directly agglutinating recombinant anti\u2010Vel IgM, M3F5S\u2010IgM, functional in manual, automated agglutination assays and flow cytometry settings. This IgM anti\u2010Vel will improve diagnostics by facilitating the identification of Vel\u2010negative blood donors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15147","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T14:00:45Z","timestamp":1548943245000},"page":"1359-1366","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a recombinant anti\u2010Vel immunoglobulin M to identify Vel\u2010negative donors"],"prefix":"10.1111","volume":"59","author":[{"given":"Marea V.E.","family":"van der Rijst","sequence":"first","affiliation":[{"name":"Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory AUMC  Amsterdam The Netherlands"},{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne N.","family":"Lissenberg\u2010Thunnissen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Ligthart","sequence":"additional","affiliation":[{"name":"Department of Immunohematology Diagnostic Services, Sanquin  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remco","family":"Visser","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Jongerius","sequence":"additional","affiliation":[{"name":"Department of Research and Lab Services National Screening Laboratory Sanquin, Sanquin  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lesley","family":"Voorn","sequence":"additional","affiliation":[{"name":"Department of Research and Lab Services National Screening Laboratory Sanquin, Sanquin  Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbera","family":"Veldhuisen","sequence":"additional","affiliation":[{"name":"Department of Immunohematology Diagnostic Services, Sanquin  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emile","family":"van den Akker","sequence":"additional","affiliation":[{"name":"Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Ellen","family":"van der Schoot","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology Sanquin Research and Landsteiner Laboratory, AUMC  Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2603"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2600"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201302466"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1976.tb04658.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13014"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002197"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40451"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.021063"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400706"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.12.013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36964"},{"key":"e_1_2_7_13_1","first-page":"e51161","article-title":"Methods for quantitative detection of antibody\u2010induced complement activation on red blood cells","author":"Meulenbroek EM","year":"2014","journal-title":"J Vis Exp"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00493-13"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.615500"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.09.014"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-211035"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12321"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8206"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/307093"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.15147","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T13:03:43Z","timestamp":1694351023000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.15147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,31]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1111\/trf.15147"],"URL":"https:\/\/doi.org\/10.1111\/trf.15147","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,31]]},"assertion":[{"value":"2018-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}