{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:30:10Z","timestamp":1780497010062,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T00:00:00Z","timestamp":1549584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600148"],"award-info":[{"award-number":["81600148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700181"],"award-info":[{"award-number":["81700181"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Cryopreservation in dimethyl sulfoxide and storage at \u221280 \u00b0C extends the shelf life of platelets to at least 2 years, allowing greater availability in rural and military areas. While cryopreserved platelets (CPPs) have been extensively characterized for coagulation and thrombin generation, reports on the mechanism of adverse reactions to CPPs transfusion are scarce. Here, we tested the hypothesis that CPPs facilitate phagocytosis by Kupffer cells and subsequently promote the inflammatory response in Kupffer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>P\u2010selectin expression, glycoprotein Ib\u03b1 clustering and phosphatidylserine (PS) surface exposure on platelets stored at 22 \u00b0C, 4 \u00b0C and\u2009\u2212\u200980 \u00b0C for 3 days were examined by flow cytometry. The phagocytosis of mepacrine\u2010labeled platelets coincubated with THP\u20101 cells was examined by flow cytometry and confocal microscopy, and the release of cytokines from THP\u20101 cells was measured by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>CPPs showed a marked enhancement of exposed PS but dramatically reduced glycoprotein Ib\u03b1 expression and clustering compared with platelets stored at 4 \u00b0C. Activation of THP\u20101 cells was stronger by CPPs than by platelets stored at 22 \u00b0C and 4 \u00b0C. CPP interference tests using annexin V and anti\u2013P\u2010selectin showed that CPPs induced increases in PS\u2010 and P\u2010selectin\u2013mediated phagocytosis, as well as secretion of the proinflammatory cytokine tumor necrosis factor\u2010\u03b1, and interleukins IL\u20101\u03b2 and IL\u20106, but a decrease in transforming growth factor\u2010\u03b2 production in THP\u20101 cells. Surface\u2010exposed PS was more effective than P\u2010selectin for the activation of THP\u20101 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>CPPs triggered PS and P\u2010selectin\u2013mediated phagocytosis by macrophages and stimulated the inflammatory response of macrophages.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15183","type":"journal-article","created":{"date-parts":[[2019,2,9]],"date-time":"2019-02-09T05:56:40Z","timestamp":1549691800000},"page":"1799-1808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cryopreserved platelets augment the inflammatory response: role of phosphatidylserine\u2010 and P\u2010selectin\u2013mediated platelet phagocytosis in macrophages"],"prefix":"10.1111","volume":"59","author":[{"given":"Jingxiang","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Health Service and Transfusion Medicine Academy of Military Medical 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