{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T14:12:03Z","timestamp":1782396723534,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T00:00:00Z","timestamp":1550620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Extending the platelet (PLT) shelf life and enhancing product safety may be achieved by combining cryopreservation and pathogen inactivation (PI). Although studied individually, limited investigations into combining these treatments has been performed. The aim of this study was to investigate the effect of PI treating PLTs before cryopreservation on in vitro PLT quality and function.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>ABO\u2010matched buffy coat\u2013derived PLTs in PLT additive solution (SSP+; Macopharma) were pooled and split to form matched pairs (n\u2009=\u20098). One unit remained untreated and the other was treated with the THERAFLEX UV\u2010Platelets System (UVC; Macopharma). For cryopreservation, 5% to 6% dimethyl sulfoxide was added to the PLTs, and they were frozen at \u221280\u00b0C. After being thawed, untreated cryopreserved PLTs (CPPs) and UVC\u2010treated CPPs (UVC\u2010CPPs) were resuspended in plasma. In vitro quality was assessed immediately after thawing and after 24\u2009hours of room temperature storage.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>UVC\u2010CPPs had lower in vitro recovery compared to CPPs. By flow cytometry, PLTs demonstrated a similar abundance of GPIX (CD42a), GPIIb (CD41a), and GPIb\u03b1 (CD42b\u2010HIP1), while the activation of GPIIb\/IIIa (PAC\u20101) was increased in UVC\u2010CPPs compared to CPPs. UVC\u2010CPPs demonstrated greater phosphatidylserine exposure (annexin\u2009V) and microparticle shedding but similar P\u2010selectin (CD62P) abundance compared to CPPs. UVC\u2010CPPs displayed similar functionality to CPPs when assessed using aggregometry, thromboelastography, and thrombin generation.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>This study demonstrates the feasibility of cryopreserving UVC\u2010PI\u2013treated PLT products. UVC\u2010PI treatment may increase the susceptibility of PLTs to damage caused during cryopreservation, but this is more pronounced during postthaw storage at room temperature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15204","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T07:50:24Z","timestamp":1550735424000},"page":"2093-2102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Cryopreservation of UVC pathogen\u2010inactivated platelets"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-055X","authenticated-orcid":false,"given":"Lauren","family":"Waters","sequence":"first","affiliation":[{"name":"Research and Development Australian Red Cross Blood Service  Sydney New South Wales Australia"},{"name":"School of Life Sciences and Proteomics Core Facility, Faculty of Science University of Technology Sydney  Sydney New South Wales 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