{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:49:53Z","timestamp":1785336593877,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The ABO transplantation barrier can be breached if antibody is reduced to low levels. Current serologic methods involve testing natural RBCs against dilutions of plasma to determine antibody levels, but these methods are poorly standardized and inherently error prone with consequent large inter\u2010 and intra laboratory variation. We evaluated the feasibility of using antigen\u2010standardized kodecytes and undiluted plasma as an alternative method for antibody measurement in patients preparing for ABO\u2010incompatible kidney transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>A panel of five kodecytes, bearing defined levels of synthetic blood group A type 2 antigen was developed (kodecyte assay) to show reaction patterns against undiluted plasma that were indicative of anti\u2010A and anti\u2010A,B levels. This panel was evaluated against the contemporary method of testing dilutions of plasma against A<jats:sub>1<\/jats:sub> cells to determine titer (A<jats:sub>1<\/jats:sub> cell assay) in both column agglutination and tube techniques. Evaluation samples included reference standards, 102 group O plus 23 group B donors, and 40 pre\u2010 and post\u2010plasmapheresis samples from five prospective ABO\u2010incompatible kidney transplant patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Comparisons between the kodecyte and A<jats:sub>1<\/jats:sub> cell assays found greater than 90% correlation for all samples. Tube and column agglutination technology platform differences were observed with A<jats:sub>1<\/jats:sub> cells and kodecytes. Discordant samples were generally found to have high ratios of IgG:IgM or vice versa.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The kodecyte assay is a simple method that requires no sample dilution, and an optimized two\u2010cell kodecyte panel is potentially capable of informing ABO\u2010incompatible kidney transplantation decisions based on antibody levels.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15247","type":"journal-article","created":{"date-parts":[[2019,3,16]],"date-time":"2019-03-16T05:52:24Z","timestamp":1552715544000},"page":"2131-2140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A standardized kodecyte method to quantify ABO antibodies in undiluted plasma of patients before ABO\u2010incompatible kidney transplantation"],"prefix":"10.1111","volume":"59","author":[{"given":"Holly","family":"Perry","sequence":"first","affiliation":[{"name":"School of Science, 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