{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:02:24Z","timestamp":1791576144978,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"am","delay-in-days":378,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T00:00:00Z","timestamp":1555113600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000182","name":"U.S. Army Medical Research and Materiel Command","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000182","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Due to high risk of septic transfusion reactions arising from bacterial contamination, US Food and Drug Administration regulations currently limit platelet storage to 5 days at room temperature (RT). However, blood culturing methods can take up to 7 days to detect bacteria, allowing transfusion of potentially contaminated units. Thus, cold storage (CS) may be a viable means of extending shelf life and improving safety.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>\n                      Platelets and fresh plasma (FP) were collected by apheresis from healthy donors, aliquoted, and challenged with\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Escherichia coli<\/jats:italic>\n                      <\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      <\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      <\/jats:styled-content>\n                      , or\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">Staphylococcus epidermidis<\/jats:styled-content>\n                      <\/jats:italic>\n                      . Aliquots were then stored at either RT or CS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Significant (p\u2009&lt;\u20090.05) bacterial growth was detected at RT for most bacteria as early as Day 1 after collection, with peak growth occurring between Days 3 and 4. Growth remained static during CS. Additionally, platelets appeared to enhance bacterial replication with growth significantly lower (p\u2009&lt;\u20090.05) in FP relative to RT\u2010stored platelets. Lactic acid promoted bacterial growth when added to FP at RT. Bacterial challenge also resulted in significantly increased platelet activation (p\u2009&lt;\u20090.05) and significantly reduced platelet function (p\u2009&lt;\u20090.05) in RT storage relative to uninfected controls by Day 5 after collection. Conversely, CS ablated bacteria growth, limited platelet metabolism, and preserved platelet function throughout the study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These data suggest that CS presents an attractive alternative to RT to both extend storage life and reduce the risk of transfusion\u2010related sepsis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.15255","type":"journal-article","created":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T14:55:42Z","timestamp":1555167342000},"page":"1479-1489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration"],"prefix":"10.1111","volume":"59","author":[{"given":"Patrick 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