{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T22:36:29Z","timestamp":1768343789646,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hellenic Society of Blood Transfusion"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Despite universal administration of erythropoiesis\u2010stimulating agents, patients with end\u2010stage renal disease (ESRD) are at high risk for presenting persistent anemia. Due to ambiguities in optimal hemoglobin targets and evidence of recombinant human erythropoietin (EPO)\u2010related toxicity, an increase in blood transfusions has been observed in chronic renal disease over the past years. The probable effects of uremic plasma on the performance of stored red blood cells (RBCs) after transfusion have not been investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Leukoreduced RBCs after short or long storage in CPD\u2010SAGM (n\u2009=\u20095) were assessed for hemolysis, surface removal signaling, reactive oxygen species (ROS) accumulation, and shape distortions before and after reconstitution with healthy (n\u2009=\u200910) or uremic plasma from ESRD patients (n\u2009=\u200920) for 24\u2009hours at physiologic temperature, by using a previously reported in vitro model of transfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Temperature and cell environment shifts from blood bag to plasma independently and in synergy affected the RBC physiology. Outcome measures at transfusion\u2010simulating conditions might not be analogous to timing of storage lesion. The uremic plasma ameliorated the susceptibility of stored RBCs to hemolysis, phosphatidylserine externalization, and ROS generation after stimulation by oxidants, but negatively affected shape homeostasis versus healthy plasma. Creatinine, uric acid, and EPO levels had correlations with the performance of stored RBCs in ESRD plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Renal insufficiency and EPO supplementation likely affect the recovery of donor RBCs and the reactivity of RBCs after transfusion by exerting both toxic and cytoprotective influences on them. ESRD patients constitute a specific recipient group that deserves further examination.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15257","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T01:29:47Z","timestamp":1553045387000},"page":"1900-1906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Recipient's effects on stored red blood cell performance: the case of uremic plasma"],"prefix":"10.1111","volume":"59","author":[{"given":"Hara T.","family":"Georgatzakou","sequence":"first","affiliation":[{"name":"Department of Biology, School of Science National &amp; Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassilis L.","family":"Tzounakas","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National &amp; Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athanassios D.","family":"Velentzas","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National &amp; Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issidora S.","family":"Papassideri","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National &amp; Kapodistrian University of Athens (NKUA)  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apostolos C.","family":"Kokkalis","sequence":"additional","affiliation":[{"name":"Chronic Hemodialysis Centre \u201cIonion\u201d  Piraeus Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantinos E.","family":"Stamoulis","sequence":"additional","affiliation":[{"name":"Hellenic National Blood Transfusion Centre  Acharnes Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasios G.","family":"Kriebardis","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, School of Health &amp; Caring Science University of West Attica (UniWA)  Egaleo Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianna H.","family":"Antonelou","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Science National &amp; 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