{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:56:58Z","timestamp":1790060218547,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T00:00:00Z","timestamp":1557100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Serologic RhD\u2010negative blood donors are tested by a method known to detect weak D antigen expression. Serology does not detect all red blood cells with RhD expression and <jats:italic>RHD<\/jats:italic> genotyping has been used to identify variant <jats:italic>RHD<\/jats:italic> alleles, which may lead to some RhD expression. The aim of this study was to determine the frequency of <jats:italic>RHD<\/jats:italic> variant alleles in serologic RhD\u2010negative blood donors at a hospital\u2010based donor center in Los Angeles.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>\n<jats:italic>RHD<\/jats:italic> genotyping of serologic RhD\u2010negative blood donors over a 20\u2010month period was performed using the Immucor <jats:italic>RHD<\/jats:italic> BeadChip assay. DNA sequencing was performed when the <jats:italic>RHD<\/jats:italic> BeadChip assay failed to assign a genotype. For <jats:italic>RHD<\/jats:italic> variants known or suspected to result in RhD expression, recipients of previous blood donations were investigated for alloimmunization.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>\n<jats:italic>RHD<\/jats:italic> genotyping was performed in 1174 RhD\u2010negative blood donors, and 1122 were genotyped for <jats:italic>RHCE<\/jats:italic> variants. Eleven donors (0.94%) harbored mutations predicted to yield RhD expression. The predicted phenotypes were, in decreasing frequency, DEL, partial, and weak D phenotypes. Anti\u2010D was not detected in 16 patients who had received blood from these donors after an average follow up of 182\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Genotyping can be used to identify donors with the potential to sensitize RhD\u2010negative recipients. In this limited study, 0.94% of serologic RhD\u2010negative blood donors were found to have variant <jats:italic>RHD<\/jats:italic> alleles that might cause alloimmunization in RhD\u2010negative recipients. To our knowledge, a study of this nature has not been reported in the United States.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15325","type":"journal-article","created":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T22:31:37Z","timestamp":1557181897000},"page":"2422-2428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>RHD<\/i> genotyping of serologic RhD\u2010negative blood donors in a hospital\u2010based blood donor center"],"prefix":"10.1111","volume":"59","author":[{"given":"Ingrid","family":"Perez\u2010Alvarez","sequence":"first","affiliation":[{"name":"Department of Pathology, Division of Transfusion Medicine University of California Irvine Medical Center  Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea","family":"Hayes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Transfusion Medicine Cedars\u2010Sinai Medical Center  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiruneh","family":"Hailemariam","sequence":"additional","affiliation":[{"name":"Immucor, Inc.  Warren New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Shin","sequence":"additional","affiliation":[{"name":"Immucor, Inc.  Warren New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler","family":"Hutchinson","sequence":"additional","affiliation":[{"name":"Immucor, Inc.  Warren New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Klapper","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Transfusion Medicine Cedars\u2010Sinai Medical Center  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.2.375"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40040428.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00579.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01975.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.02046.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2014.02.011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2011.01129.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14425"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12582"},{"key":"e_1_2_7_11_1","unstructured":"Blood grouping reagents formulated for use in automated systems Beckman Coulter PK Systems [Internet] [cited 2018 October 6]. 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