{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:20:31Z","timestamp":1762431631263,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The diagnosis of autoimmune hemolytic anemia (AIHA) can be challenging since the direct antiglobulin test (DAT) has been reported to be falsely negative in 3%\u201011% of cases. In children with anemia, laboratory and\/or clinical evidence of hemolysis and a negative DAT, clinicians should consider further specialized testing to confirm AIHA to accurately diagnose and treat this uncommon pediatric entity.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>A retrospective chart review was undertaken at a large tertiary care academic pediatric hematology practice to describe our experience with DAT\u2010negative AIHA.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>From January 1, 2010 through August 1, 2016, 10 children were described who had clinical and laboratory evidence of AIHA, a negative DAT, and further specialized serologic testing confirming this diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This case series highlights the need for further serologic workup when a child's clinical presentation is highly consistent with AIHA despite a negative DAT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15350","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T12:04:29Z","timestamp":1558440269000},"page":"2528-2531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A case series of pediatric patients with direct antiglobulin test negative autoimmune hemolytic anemia"],"prefix":"10.1111","volume":"59","author":[{"given":"Jonathan","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Oncology St. Jude Children's Research Hospital  Memphis Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Transfusion Medicine Stanford University School of Medicine  Stanford California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Department of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2325-3388","authenticated-orcid":false,"given":"Anupama","family":"Narla","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Hematology &amp; 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