{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T23:39:26Z","timestamp":1776987566571,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T00:00:00Z","timestamp":1574380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Cellular therapies using engineered T cells, haploidentical transplants, and autologous gene therapy are increasing. Specified CD3+ or high CD34+ doses are typically required for subsequent manufacturing, manipulation, or CD34+ selection. Simple, practical, and reliable lymphocyte and hematopoietic progenitor cell (HPC) collection algorithms accounting for subsequent CD34+ selection have not been published.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>In this analysis of 15 haploidentical donors undergoing tandem lymphocyte and HPC collections, we validated one\u2010step, practical prediction algorithms (Appendix <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#trf15576-supitem-0001\">S1<\/jats:ext-link>, available as supporting information in the online version of this paper) that use conservative facility\u2010specific collection efficiencies, CD34+ selection efficiency, and donor\u2010specific peripheral counts to reliably achieve the target CD3+ and CD34+ product doses. These algorithms expand on our previously published work regarding predictive HPC collection algorithms.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Ninety\u2010three percent of lymphocyte and 93% of CD34+ collections achieved the final target CD3+ and CD34+ product dose when our algorithm\u2010calculated process volumes were used. Linear regression analysis of our algorithms for CD3+, preselection CD34+, and postselection CD34+ showed statistically significant models with R<jats:sup>2<\/jats:sup> of 0.80 (root mean square error [RMSE], 31.3), 0.72 (RMSE, 385.7), and 0.56 (RMSE, 326.0), respectively, all with p values less than 0.001.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Because achievement of CD3+ or CD34+ dose targets may be critical for safety and efficacy of cell therapies, these simple, practical, and reliable prediction algorithms for lymphocyte and HPC collections should be very useful for collection facilities.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15576","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T13:40:26Z","timestamp":1574430026000},"page":"133-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Validation of simple prediction algorithms to consistently achieve CD3+ and postselection CD34+ targets with leukapheresis"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3841-8133","authenticated-orcid":false,"given":"Edward J.","family":"Yoon","sequence":"first","affiliation":[{"name":"Clinical Services New York Blood Center  New York New York"},{"name":"Temple University Hospital  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Clinical Services New York Blood Center  New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rona S.","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Clinical Services New York Blood Center  New York New York"},{"name":"Lindsley F. Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel A.","family":"Brochstein","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\u2010Oncology Northwell Health  New Hyde Park New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Nandi","sequence":"additional","affiliation":[{"name":"Lindsley F. Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"Sachais","sequence":"additional","affiliation":[{"name":"Clinical Services New York Blood Center  New York New York"},{"name":"Lindsley F. 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