{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:17Z","timestamp":1747188437003,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T00:00:00Z","timestamp":1574640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Microorganism contamination of platelets results in a high risk of transfusion\u2010related sepsis. Here, the ability of culture vials (BD BACTEC Platelet Aerobic\/F and Platelet Anaerobic\/F vials, Becton, Dickinson and Company) to detect microorganisms in leukoreduced apheresis platelets (LRAPs) and leukoreduced whole blood platelet concentrates (LRWBPCs) was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>LRAPs or LRWBPCs were inoculated into Aerobic\/F and Anaerobic\/F vials and placed in a blood culturing system (BD BACTEC FX System, Becton, Dickinson and Company) for growth\/monitoring over 7\u2009days to detect preexisting contamination during false\u2010positive testing. Subsequently, platelets were seeded with microorganisms at approximately 10 CFU\/mL or approximately 1 CFU\/mL to simulate contamination. Aerobic\/F and Anaerobic\/F vials were inoculated with platelets (sets of 12). Microorganism growth was detected in the BACTEC FX instrument over 7\u2009days. Overall, 2925 vials were tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Of the 1905 vials included in the microorganism detection phase, 63 (3.3%) Aerobic\/F and 16 (0.8%) Anaerobic\/F vials were both BACTEC FX and subculture negative. From the remaining 1827 vials, two (0.1%) Anaerobic\/F vials were false positive; no false positives were observed in Aerobic\/F vials, and no false negatives occurred in either vial type. Of the remaining 1825 vials (99.9%), 955 Aerobic\/F and 870 Anaerobic\/F vials were true positives. The mean\u2010time\u2010to\u2010detection range was 8.5 to 77\u2009hours. All true\u2010positive Aerobic\/F and Anaerobic\/F vials showed 100% agreement with subculture for positive identification of seeded microorganisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Aerobic\/F and Anaerobic\/F vials facilitate contamination detection in LRAPs and LRWBPCs down to approximately 1 CFU\/mL. These results support the use of Aerobic\/F and Anaerobic\/F vials for quality control testing of platelets before transfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15583","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T06:59:53Z","timestamp":1574751593000},"page":"126-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A dual\u2010center evaluation of platelet culture vials to detect the presence of microorganisms in platelets"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1627-4818","authenticated-orcid":false,"given":"Gerald","family":"Denys","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anagha","family":"Tulpule","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Roth","sequence":"additional","affiliation":[{"name":"Becton, Dickinson and Company BD Life Sciences \u2013 Diagnostic Systems  Sparks Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patty","family":"Warns","sequence":"additional","affiliation":[{"name":"Becton, Dickinson and Company BD Life Sciences \u2013 Diagnostic Systems  Sparks Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiffany","family":"Collins","sequence":"additional","affiliation":[{"name":"Becton, Dickinson and Company BD Life Sciences \u2013 Diagnostic Systems  Sparks Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Mindel","sequence":"additional","affiliation":[{"name":"Becton, Dickinson and Company BD Life Sciences \u2013 Diagnostic Systems  Sparks Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1972.tb16342.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7326\/M14-1589"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14165"},{"key":"e_1_2_9_5_1","unstructured":"Food and Drug Administration. Bacterial risk control strategies for blood collection establishments and transfusion services to enhance the safety and availability of platelets for transfusion. Draft guidance for industry. 2018. Available from:https:\/\/www.fda.gov\/media\/123448\/download."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.195-204.2005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02348.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.45"},{"key":"e_1_2_9_9_1","unstructured":"American Association of Blood Banks. Standards for blood banks and transfusion. [cited 2018 Apr 1]. Available from:http:\/\/www.aabb.org\/sa\/standards\/Pages\/standards-programs.aspx. 31st ed."},{"key":"e_1_2_9_10_1","unstructured":"Food and Drug Administration. Control of bacterial contamination of platelets 21CFR 606.145.2018. Available from:https:\/\/www.govregs.com\/regulations\/21\/606.145"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx167"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-655944"},{"key":"e_1_2_9_13_1","unstructured":"Blood Products Advisory Committee Meeting. FDA White Oak Campus Silver Springs MD. 2018. Topic I: strategies to control the risk of bacterial contamination in platelets for transfusion. Available from:https:\/\/www.fda.gov\/downloads\/AdvisoryCommittees\/CommitteesMeetingMaterials\/BloodVaccinesandOtherBiologics\/BloodProductsAdvisoryCommittee\/UCM611834.pdf."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-11-833996"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03308.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2212-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000357651"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14911"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04343.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-12-685198"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-012799"},{"volume-title":"StatPearls","year":"2019","author":"Lotterman S","key":"e_1_2_9_22_1"},{"key":"e_1_2_9_23_1","first-page":"e5","article-title":"Bacterial contamination of platelet concentrates: pathogen detection and inactivation methods","volume":"1","author":"V\u00e9dy D","year":"2009","journal-title":"Hematol Rev"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000330466"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1586\/ehm.11.53"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000330312"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01248.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2009.01221.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03311.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142398"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis1021"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01958-10"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02260-10"},{"key":"e_1_2_9_34_1","first-page":"47","article-title":"Prevalence of bacterial contamination when using a diversion pouch during blood collection: a single center study in Malaysia","volume":"21","author":"Jumaah N","year":"2014","journal-title":"Malays J Med Sci"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.15583","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T14:09:27Z","timestamp":1694095767000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.15583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,25]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/trf.15583"],"URL":"https:\/\/doi.org\/10.1111\/trf.15583","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2019,11,25]]},"assertion":[{"value":"2019-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}