{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T08:28:35Z","timestamp":1771921715488,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000008","name":"Health Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Vancomycin\u2010resistant enterococci (VRE) are antibiotic\u2010resistant organisms associated with both colonization and serious life\u2010threatening infection in health care settings. Contamination of platelet concentrates (PCs) with <jats:italic>Enterococcus<\/jats:italic> can result in transfusion\u2010transmitted infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>CASE PRESENTATION<\/jats:title><jats:p>This report describes the investigation of a septic transfusion case involving a 27\u2010year\u2010old male patient with relapsed acute leukemia who was transfused with a 5\u2010day\u2010old buffy coat PC pool and developed fever and rigors.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>Microbiology testing and pulse\u2010field gel electrophoresis (PFGE) was done on patient blood cultures obtained from peripheral and central lines. Microbiology and molecular testing were also performed on the remaining posttransfusion PC pool, which was refrigerated for 24\u2009hours before microbiology testing. Red blood cell (RBC) and plasma units associated with the implicated PCs were screened for microbial contamination. Patient blood cultures obtained from peripheral and central lines yielded vancomycin\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>Enterococcus faecium<\/jats:italic><\/jats:styled-content>. Gram stain of a sample from the platelet pool was negative but coagulase\u2010negative <jats:italic>Staphylococcus<\/jats:italic> (CNST) and VRE were isolated on culture. Antibiotic sensitivity and PFGE profiles of several VRE isolates from the patient before and after transfusion, and the PC pool, revealed that all were closely related. Associated RBC and plasma components tested negative for microbial contamination.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Microbiological and molecular investigations showed a relationship between VRE isolated from the patient before and after transfusion, and therefore it is postulated that a patient\u2010to\u2010PC retrograde contamination (from either blood or skin) occurred. As the CNST isolated from the PC pool was not isolated from patient samples, its implication in the transfusion event is unknown.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15644","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T11:30:46Z","timestamp":1576841446000},"page":"430-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A suspected septic transfusion reaction associated with posttransfusion contamination of a platelet pool by vancomycin\u2010resistant <scp><i>Enterococcus faecium<\/i><\/scp>"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-1109","authenticated-orcid":false,"given":"Steven J.","family":"Drews","sequence":"first","affiliation":[{"name":"Canadian Blood Services  Edmonton Alberta Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Lesley","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Detsky","sequence":"additional","affiliation":[{"name":"Mount Sinai Hospital  Toronto Ontario Canada"},{"name":"Interdepartmental Division of Critical Care Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanis","family":"Distefano","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ancaster Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesario","family":"Ilagan","sequence":"additional","affiliation":[{"name":"Mount Sinai Hospital  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangeeta","family":"Mehta","sequence":"additional","affiliation":[{"name":"Mount Sinai Hospital  Toronto Ontario Canada"},{"name":"Interdepartmental Division of Critical Care Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"McGeer","sequence":"additional","affiliation":[{"name":"Mount Sinai Hospital  Toronto Ontario Canada"},{"name":"Department of Medicine and Laboratory Medicine and Pathobiology University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Shehata","sequence":"additional","affiliation":[{"name":"Mount Sinai Hospital  Toronto Ontario Canada"},{"name":"Department of Medicine and Laboratory Medicine and Pathobiology University of Toronto  Toronto Ontario Canada"},{"name":"Canadian Blood Services  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Skeate","sequence":"additional","affiliation":[{"name":"Department of Medicine and Laboratory Medicine and Pathobiology University of Toronto  Toronto Ontario Canada"},{"name":"Canadian Blood Services  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-4987","authenticated-orcid":false,"given":"Sandra","family":"Ramirez\u2010Arcos","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"},{"name":"Department of Biochemistry, Microbiology, and Immunology University of Ottawa  Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.14745\/ccdr.v42i11a03"},{"key":"e_1_2_7_3_1","unstructured":"TacconelliE. 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