{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T22:48:49Z","timestamp":1765493329492,"version":"3.43.0"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007052","name":"Memorial Sloan-Kettering Cancer Center","doi-asserted-by":"publisher","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}],"id":[{"id":"10.13039\/100007052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA008748","P30"],"award-info":[{"award-number":["CA008748","P30"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Patients with congenital haptoglobin deficiency can develop anti\u2010haptoglobin antibodies after exposure to blood products, and they can suffer from life\u2010threatening anaphylactic transfusion reactions. Here, we present a case of a 57\u2010year\u2010old Chinese male with myelodysplastic syndrome who manifested an anaphylactic transfusion reaction during the transfusion of platelets. The only abnormality detected during his reaction laboratory workup was an undetectable haptoglobin level in the absence of evidence of hemolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Surface plasmon resonance (SPR) was explored as a method to be able to detect the presence of anti\u2010haptoglobin antibodies in serum. First, haptoglobin was immobilized to the surface of an SPR sensor chip. The patient's serum sample was injected, and the binding response was monitored in real time. Serum samples from five healthy volunteers were used as negative controls. Binding specificity was assessed in competition experiments using soluble haptoglobin. Anti\u2010IgG, \u2010IgA, \u2010IgM, \u2010IgD and \u2010IgE antibodies were used to identify the antibody isotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>An IgG anti\u2010haptoglobin antibody was detected in the patient's serum with SPR.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>SPR provided a rapid, readily available method for the detection of an IgG anti\u2010haptoglobin antibody in an anhaptoglobinemic individual. This confirmed the underlying etiology of the anaphylactic nonhemolytic transfusion reaction and justified the necessity of stringently washed cellular products for all future transfusions and strong caution for future use of plasma\u2010containing products.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15657","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T01:24:40Z","timestamp":1579829080000},"page":"682-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A novel method for the laboratory workup of anaphylactic transfusion reactions in haptoglobin\u2010deficient patients"],"prefix":"10.1111","volume":"60","author":[{"given":"Katie L.","family":"Thoren","sequence":"first","affiliation":[{"name":"Department\u2009of Laboratory Medicine Memorial\u2009Sloan Kettering Cancer Center  New\u2009York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott T.","family":"Avecilla","sequence":"additional","affiliation":[{"name":"Department\u2009of Laboratory Medicine Memorial\u2009Sloan Kettering Cancer Center  New\u2009York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Klimek","sequence":"additional","affiliation":[{"name":"Leukemia Service Department of Medicine, Memorial\u2009Sloan Kettering Cancer Center  New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3435-175X","authenticated-orcid":false,"given":"Cheryl","family":"Goss","sequence":"additional","affiliation":[{"name":"Department\u2009of Laboratory Medicine Memorial\u2009Sloan Kettering Cancer Center  New\u2009York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572008000400002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/42.10.1589"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.9.3524"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/301701"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.4.1138.004k27_1138_1143"},{"key":"e_1_2_9_7_1","unstructured":"National Healthcare Safety Network Biovigilance Component Hemovigilance Module Surveillance Protocol. [cited 2019 Dec 2]. 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