{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:04:30Z","timestamp":1792638270213,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Anti\u2010CD36s, developing after transfusion or during pregnancy, play an important role in immune\u2010mediated bleeding disorders among Asian populations. Currently, little is known about the clinical relevance of anti\u2010CD36. Here, we aimed to determine the frequency of CD36 deficiency in Thais by analyzing CD36 expression on cell surfaces and in plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>The expression and deficiency of CD36 on platelets and monocytes were determined by flow cytometry. Mutations in the CD36 gene were analyzed by nucleotide sequencing. Soluble CD36 (sCD36) in plasma was quantified with enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Fifteen of 700 blood donors (2.14%) were identified as CD36 deficient. The frequencies of Type I and II CD36 deficiency were 0.43% and 1.71%, respectively. Type I individuals exhibited c.1163A\u2009&gt;\u2009T, c.429\u2009+\u20094insG, and c.1156C\u2009&gt;\u2009T. Type II individuals exhibited c.879\u2009T\u2009&gt;\u2009C, c.329\u2010330delAC, c.818\u2009+\u2009108delAACT, c.1125\u2009+\u200913C\u2009&gt;\u2009A, and c.1163A\u2009&gt;\u2009T. CD36 on donor platelets (n = 685) showed a wide distribution of expression levels (mean fluorescence intensity, 16.71\u2009\u00b1\u20098.68). In the normal phenotype (n = 14), sCD36 concentration was 58.84\u2009\u00b1\u200911.68\u2009ng\/mL, which was significantly correlated with platelet CD36 expression (r<jats:sup>2<\/jats:sup> =\u20090.8551). In Type II\u2013deficient individuals (n = 6), a similar sCD36 concentration was detected (53.67\u2009\u00b1\u20098.17\u2009ng\/mL). However, sCD36 could not be detected in Type I individuals (n = 3).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>CD36 Type I deficiency was found, indicating the potential for immune\u2010mediated platelet disorders in Thais. However, the underlying mutations differed from those reported in Japan and China. Interestingly, sCD36 could not be detected in plasma of Type I\u2013deficient individuals. This finding may lead to the use of plasma to identify individuals at risk and to allow screening of large cohorts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15737","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T07:43:30Z","timestamp":1583307810000},"page":"847-854","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Frequency of CD36 deficiency in Thais analyzed by quantification of CD36 on cell surfaces and in plasma"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3876-6426","authenticated-orcid":false,"given":"Roongaroon","family":"Phuangtham","sequence":"first","affiliation":[{"name":"Biomedical Sciences Program Graduate School, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sentot","family":"Santoso","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology and Transfusion Medicine Justus Liebig University Giessen  Giessen Germany"},{"name":"Guangzhou Blood Centre Institute of Blood Transfusion  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanvit","family":"Leelayuwat","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Transfusion Sciences, Faculty of Associated Medical Sciences Khon Kaen University  Khon Kaen Thailand"},{"name":"The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharee","family":"Komvilaisak","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine Khon Kaen University  Khon Kaen 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