{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T09:29:05Z","timestamp":1780824545188,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007813","name":"Center for AIDS Research, University of Washington","doi-asserted-by":"publisher","award":["P30 AI050409"],"award-info":[{"award-number":["P30 AI050409"]}],"id":[{"id":"10.13039\/100007813","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In 2014, passive immunization by transfusion of Ebola convalescent plasma (ECP) was considered for treating patients with acute Ebola virus disease (EVD). Early Ebola virus (EBOV) seroconversion confers a survival advantage in natural infection, hence transfusion of ECP plasma with high levels of neutralizing EBOV antibodies is a potential passive immune therapy. Techniques to reduce the risk of other transfusion\u2010transmitted infections (TTIs) are warranted as recent ECP survivors are ineligible as routine blood donors. As part of an ongoing clinical trial to evaluate the safety and effectiveness of ECP, the impact of amotosalen\/UVA pathogen reduction technology (PRT) on EBOV antibody characteristics was examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Serum and plasma samples were collected from EVD\u2010recovered subjects at multiple timepoints and evaluated by ELISA for antibodies to recombinant EBOV glycoprotein (GP) and irradiated whole EBOV antigen, as well as for EBOV microneutralization, classic plaque reduction neutralization test (PRNT) and EBOV pseudovirion neutralization assay (PsVNA) activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Six subjects donated 40 individual ECP units. Substantial antibody titers and neutralizing activity results were demonstrated but were generally lower for the ACD plasma samples compared to the serum samples. Anti\u2010EBOV titers by all assays remained essentially unchanged after PRT.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Treatment of ECP with PRT to reduce the risk of TTI did not significantly reduce EBOV IgG antibody titers or neutralizing activity. Although ECP was used in the treatment of repatriated patients, no PRT units from this study were transfused to EVD patients. This inventory of PRT\u2010treated ECP is currently available for future clinical evaluation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15739","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T12:39:57Z","timestamp":1583325597000},"page":"1024-1031","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Characterization of Ebola convalescent plasma donor immune response and psoralen treated plasma in the United States"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-2074","authenticated-orcid":false,"given":"Christina L.","family":"Dean","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay W.","family":"Hooper","sequence":"additional","affiliation":[{"name":"Division of Virology US Army Medical Research Institute for Infectious Diseases  Fort Detrick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Dye","sequence":"additional","affiliation":[{"name":"Division of Virology US Army Medical Research Institute for Infectious Diseases  Fort Detrick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha E.","family":"Zak","sequence":"additional","affiliation":[{"name":"Division of Virology US Army Medical Research Institute for Infectious Diseases  Fort Detrick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Koepsell","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8615-9869","authenticated-orcid":false,"given":"Laurence","family":"Corash","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-4744","authenticated-orcid":false,"given":"Richard J.","family":"Benjamin","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Kwilas","sequence":"additional","affiliation":[{"name":"Division of Virology US Army Medical Research Institute for Infectious Diseases  Fort Detrick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Bonds","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Winkler","sequence":"additional","affiliation":[{"name":"Instrumentation Laboratory  Bedford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen S.","family":"Kraft","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.su6503a2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr1513109"},{"key":"e_1_2_7_4_1","first-page":"247","article-title":"Ebola haemorrhagic fever in Sudan, 1976. 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