{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:04:51Z","timestamp":1747188291627,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Viscoelastic tests (VETs) are used widely to monitor hemostasis in settings such as cardiac surgery. There has also been renewed interest in cold stored platelets (CSPs) to manage bleeding in this setting. CSPs are reported to have altered hemostatic properties compared to room temperature platelets (RTPs), including activation of GPIIb\/IIIa. We investigated whether the functional differences between CSP and RTP affected the performance of the PlateletMapping VET on the TEG 5000 and 6s analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHOD<\/jats:title><jats:p>Platelet concentrates were divided equally into CSP (stored at 4\u00b0C\u2009\u00b1\u20092\u00b0C) and RTP (stored at 22\u00b0C\u2009\u00b1\u20092\u00b0C) fractions. Whole blood was treated to induce platelet dysfunction (WBIPD) by incubating with anti\u2010platelet drugs (1.0 \u03bcM ticagrelor and 10 \u03bcM aspirin) or by simulating cardiopulmonary bypass. WBIPD samples were then mixed with 20% by volume of CSPs or RTPs to model platelet transfusion before analysis using the PlateletMapping VET.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Addition of CSPs to WBIPD increased the PlateletMapping MA<jats:sub>FIBRIN<\/jats:sub> and MA<jats:sub>ADP<\/jats:sub> parameters with the TEG 5000 analyzer (both p\u2009&lt;\u20090.0001 compared to addition of buffer alone). This effect was not observed with RTPs. The differential effect of CSPs on the MA<jats:sub>FIBRIN<\/jats:sub> corrected after pre\u2010incubation with the GPIIb\/IIIa antagonist tirofiban and was quantitatively less with the PlateletMapping test for the TEG 6s analyzer which contains the GPIIb\/IIa antagonist abciximab.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>The PlateletMapping MA<jats:sub>FIBRIN<\/jats:sub> and MA<jats:sub>ADP<\/jats:sub> test results may be misleadingly high with CSPs, particularly with the TEG 5000 analyzer, most likely due to constitutive activation of GPIIb\/IIIa on CSPs during storage. TEG PlateletMapping results should be interpreted with caution following CSP transfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15753","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T13:54:16Z","timestamp":1591019656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["TEG <scp>PlateletMapping<\/scp> assay results may be misleading in the presence of cold stored platelets"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4106-2286","authenticated-orcid":false,"given":"Thomas G.","family":"Scorer","sequence":"first","affiliation":[{"name":"Centre of Defence Pathology Royal Centre of Defence Medicine  Birmingham UK"},{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"},{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucy","family":"FitzGibbon","sequence":"additional","affiliation":[{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riyaad","family":"Aungraheeta","sequence":"additional","affiliation":[{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umang","family":"Sharma","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grantham C.","family":"Peltier","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colby S.","family":"McIntosh","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-9780","authenticated-orcid":false,"given":"Kristin M.","family":"Reddoch\u2010Cardenas","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Meyer","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"},{"name":"Division of Pediatric Critical Care, Department of Pediatrics University of Texas Health Science Center  San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew P.","family":"Cap","sequence":"additional","affiliation":[{"name":"Coagulation and Blood Research U.S. Army Institute of Surgical Research  Fort Sam Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D.","family":"Mumford","sequence":"additional","affiliation":[{"name":"School of Cellular and Molecular Medicine University of Bristol  Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"National Institute for Health and Care Excellence (NICE). Diagnostics guidance 13: detecting managing and monitoring haemostasis: viscoelastometric point\u2010of\u2010care testing (ROTEM TEG and Sonoclot systems). 2014 [cited 2019 Sept 21]. Available from:https:\/\/www.nice.org.uk\/guidance\/dg13."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta19580"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aex100"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13580"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.15524"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7020539"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0000000000002037"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365513.2017.1325000"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-9560-5-3"},{"key":"e_1_2_7_11_1","unstructured":"TEG system. Haemonetics. [cited 2019 Sept 21]. Available from:https:\/\/teg.haemonetics.com\/en."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2018.12.012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.10363.1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12380"},{"key":"e_1_2_7_15_1","article-title":"Targeting hemorrhage: alternative storage of platelets for hemostatic transfusion","volume":"130","author":"Cap AP","year":"2017","journal-title":"Blood"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13504"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000082"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14751"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13723"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13511"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03917.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000493"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-99-111428"},{"key":"e_1_2_7_24_1","unstructured":"JPAC. Guidelines for the blood transfusion services in the UK: 8th edition red book. 2013 [cited 2019 Sept 21]. Available from:https:\/\/www.transfusionguidelines.org\/red-book."},{"key":"e_1_2_7_25_1","unstructured":"Blood safety and quality regulations 2005 UK Statutory Instrument No.50 2005[cited 2019 Sept 21]. Available from:http:\/\/www.legislation.gov.uk\/uksi\/2005\/50\/contents\/made."}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15753","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.15753","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15753","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T09:52:19Z","timestamp":1693993939000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.15753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":24,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1111\/trf.15753"],"URL":"https:\/\/doi.org\/10.1111\/trf.15753","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"2019-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}