{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:52:54Z","timestamp":1777524774193,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T00:00:00Z","timestamp":1584489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Our previous study showed that ultraviolet C (UVC) from xenon (Xe) flash without any photoreactive compounds inactivated bacteria in platelet concentrates (PCs) with less damage to platelets (PLTs) as compared with Xe flash containing ultraviolet A, ultraviolet B, and visible light. Here, we report a UVC irradiation system for PCs under flow conditions consisting of a flow path\u2013irradiation sheet, a peristaltic pump, and a collection bag.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Platelet concentrates containing Ringer\u02bcs solution (R\u2010PCs) inoculated with bacteria were injected into a flow path sheet using a peristaltic pump, being irradiated with UVC from Xe flash. The quality of the irradiated PCs containing platelet additive solution (PAS\u2010PCs) was assessed based on PC variables, PLT surface markers, and aggregation ability.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Streptococcus dysgalactiae<\/jats:italic><\/jats:styled-content> (12 tests) and <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content> (11) were all negative on bacterial culture, while <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> (12) and <jats:styled-content style=\"fixed-case\"><jats:italic>Klebsiella pneumoniae<\/jats:italic><\/jats:styled-content> (14) grew in one and two R\u2010PCs, respectively. <jats:styled-content style=\"fixed-case\"><jats:italic>Bacillus cereus<\/jats:italic><\/jats:styled-content> spores were inactivated in 7 of 12 R\u2010PCs. PC variables became significantly different between irradiated and nonirradiated PAS\u2010PCs. P\u2010selectin, first procaspase\u2010activating compound (PAC\u20101) binding, and phosphatidylserine increased by irradiation. Aggregability stimulated by adenosine diphosphate, collagen, or thromboxane A<jats:sub>2<\/jats:sub> increased in the irradiated PAS\u2010PCs, while that by thrombin became smaller compared with nonirradiated controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This newly developed system inactivated bacteria including spores in R\u2010PCs. PAS\u2010PCs irradiated by this system retained acceptable in vitro quality and aggregability. Usage of a peristaltic pump instead of agitator during irradiation may enable this system to be directly combined with an apheresis blood cell separator.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15757","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T21:45:16Z","timestamp":1584567916000},"page":"1050-1059","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Flow path system of ultraviolet C irradiation from xenon flash to reduce bacteria survival in platelet products containing a platelet additive solution"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0881-0089","authenticated-orcid":false,"given":"Hideki","family":"Abe","sequence":"first","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimika","family":"Endo","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-1955","authenticated-orcid":false,"given":"Masayuki","family":"Shiba","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Niibe","sequence":"additional","affiliation":[{"name":"Comet Co. LTD  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Miyata","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4660-6839","authenticated-orcid":false,"given":"Masahiro","family":"Satake","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,18]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Infectious cases that were probably related to transfused blood components (2017) [monograph on the Internet]. Japanese Red Cross Society; 2018. [cited June 10 2019]. 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