{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T00:42:54Z","timestamp":1792975374664,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Cryopreserved platelets are phenotypically and functionally different to conventionally stored platelets. Calcium may be released from internal stores during the freeze\u2013thaw process, initiating signaling events which lead to these alterations. It was hypothesized that the addition of a calcium chelator prior to cryopreservation may mitigate some of these changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Buffy coat\u2013derived platelets that had been pooled and split were tested fresh and following cryopreservation (n = 8 per group). Platelets were cryopreserved using 5%\u20106% dimethylsulfoxide (DMSO) or were supplemented with increasing concentrations of the internal calcium chelator, BAPTA\u2010AM (100\u2009\u03bcM, 200\u2009\u03bcM, or 400\u2009\u03bcM), prior to storage at \u221280\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Supplementation of platelets with BAPTA\u2010AM prior to freezing improved platelet recovery in a dose response manner (400\u2009\u03bcM: 84\u2009\u00b1\u20092%) compared to standard DMSO cryopreserved platelets (70\u2009\u00b1\u20094%). There was a loss of GPIb\u03b1, GPVI, and GPIIb\/IIIa receptors on platelets following cryopreservation, which was rescued when platelets were supplemented with BAPTA\u2010AM (400\u2009\u03bcM: p\u2009&lt;\u20090.0001 for all). Platelet activation markers, such as phosphatidylserine and P\u2010selectin, were externalized on platelets following cryopreservation. However, the addition of BAPTA\u2010AM significantly reduced the increase of these activation markers on cryopreserved platelets (400\u2009\u03bcM: p\u2009&lt;\u20090.0001 for both). Both cryopreserved platelet groups exhibited similar functionality as assessed by thromboelastography, forming clots at a faster rate than fresh platelets.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>This study demonstrates that calcium plays a crucial role in mediating cryopreservation\u2010induced damage to frozen platelets. The addition of the calcium chelator, BAPTA\u2010AM, prior to cryopreservation reduces this damage.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15799","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T11:39:42Z","timestamp":1587555582000},"page":"1552-1563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Calcium chelation: a novel approach to reduce cryopreservation\u2010induced damage to frozen platelets"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4740-055X","authenticated-orcid":false,"given":"Lauren","family":"Waters","sequence":"first","affiliation":[{"name":"Research and Development Australian Red Cross Lifeblood (formerly the Australian Red Cross Blood Service)  Alexandria New South Wales Australia"},{"name":"School of Life Sciences and Proteomics Core Facility, Faculty of Science University of Technology Sydney  Sydney New South 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