{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:19:02Z","timestamp":1788347942460,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Strategies to reduce platelet (PLT) bacterial contamination include donor screening, skin disinfection, sample diversion, bacterial culture, pathogen reduction (PR), and day\u2010of\u2010transfusion tests. We report bacterial sepsis following a pathogen\u2010reduced PLT transfusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case Report<\/jats:title>\n                    <jats:p>\n                      An adult male with relapsed acute lymphoblastic leukemia was successfully treated for central catheter\u2013associated\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      bacteremia. A peripherally inserted central catheter (PICC) was placed. Chills, rigors, and flushing developed immediately after PICC\u2010infused pathogen\u2010reduced PLTs, progressing to septic shock requiring intensive care management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PICC and peripheral blood (PB), transfused bag saline flushes (TBFs), environmental samples, and the pathogen\u2010reduced untransfused co\u2010component (CC) were cultured. Plasma metagenomic and bacterial isolate whole\u2010genome sequencing; PLT mitochondrial DNA (mtDNA) testing of untransfused CC and TBF; CC testing for amotosalen (S\u201059)\/S\u201059 photoproducts; isolate PR studies (INTERCEPT); and TBF polymerase chain reaction for recipient Y\u2010chromosome DNA were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PB and PICC cultures grew\n                      <jats:italic>Acinetobacter calcoaceticus\/baumannii<\/jats:italic>\n                      complex (ACBC). TBF was gram\u2010positive; mass spectrometry identified ACBC and\n                      <jats:italic>Staphylococcus saprophyticus<\/jats:italic>\n                      (SS). CC Gram stain and cultures were negative. Environmental cultures, some done after decontamination, were ACBC\/SS negative. Posttransfusion patient plasma and TBF ACBC sequences were genetically identical. No Y\u2010chromosome signal was detected in TBF. S\u201059 photoproducts and evidence of mtDNA amplification inhibition were found in the CC. Spiking PR studies showed &gt;5.9\u2010log inactivation for both isolates. Donor skin cultures for\n                      <jats:italic>Acinetobacter<\/jats:italic>\n                      were negative.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CC sterility, PR studies, residual S\u201059 photoproducts, and mtDNA amplification inhibition suggest successful PR. Unidentified environmental sources and inherent or acquired bag defects may have contributed to postmanufacturing pathogen\u2010reduced PLT contamination.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.15951","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T08:44:10Z","timestamp":1596271450000},"page":"1960-1969","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Sepsis from an apheresis platelet contaminated with\n                    <scp>\n                      <i>Acinetobacter calcoaceticus\/baumannii<\/i>\n                    <\/scp>\n                    complex bacteria and\n                    <scp>\n                      <i>Staphylococcus saprophyticus<\/i>\n                    <\/scp>\n                    after pathogen reduction"],"prefix":"10.1111","volume":"60","author":[{"given":"Joy L.","family":"Fridey","sequence":"first","affiliation":[{"name":"Southern California Region American Red Cross  Pomona California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5865-6366","authenticated-orcid":false,"given":"Susan L.","family":"Stramer","sequence":"additional","affiliation":[{"name":"Scientific Affairs American Red Cross  Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Nambiar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5752-4997","authenticated-orcid":false,"given":"Morvarid","family":"Moayeri","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Bakkour","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Langelier","sequence":"additional","affiliation":[{"name":"Chan Zuckerberg Biohub University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Crawford","sequence":"additional","affiliation":[{"name":"Chan Zuckerberg Biohub University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thea","family":"Lu","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4213-5013","authenticated-orcid":false,"given":"Marion C.","family":"Lanteri","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Kamm","sequence":"additional","affiliation":[{"name":"Chan Zuckerberg Biohub University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5720-4862","authenticated-orcid":false,"given":"Stephen J.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Transfusion Innovation American Red Cross  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-4744","authenticated-orcid":false,"given":"Richard J.","family":"Benjamin","sequence":"additional","affiliation":[{"name":"Cerus Corporation  Concord California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1446-125X","authenticated-orcid":false,"given":"Michael P.","family":"Busch","sequence":"additional","affiliation":[{"name":"Vitalant Research Institute  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"13","volume-title":"Standards for blood banks and transfusion services","author":"Fridey JL","year":"2003"},{"key":"e_1_2_8_3_1","first-page":"11","volume-title":"Standards for blood banks and transfusion services","author":"Carson TH","year":"2011"},{"key":"e_1_2_8_4_1","unstructured":"US Food and Drug Administration.Bacterial Risk Control Strategies for Blood Collection Establishments and Transfusion Services to Enhance the Safety and Availability of Platelets for Transfusion. 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