{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:11:38Z","timestamp":1762459898022,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T00:00:00Z","timestamp":1595635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Antibodies against human platelet antigens (HPAs) cause thrombocytopenias. It is thus important to know the frequency of \u201cb\u201d allotypes in each HPA system for the diagnosis and treatment of anti\u2010HPA antibody\u2010mediated thrombocytopenia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Genomic DNA was extracted from peripheral blood cells obtained from 2170 blood donors in Japan and was subjected to high\u2010resolution melt (HRM) analysis using polymerase chain reaction for each of the HPA genes, using 23 primer pairs. For genotyping, the resulting amplicons were classified based on their HRM curves. In some cases, direct sequence analysis was performed after HRM analysis to determine nucleotide substitutions. In cases where amino acid substitutions were predicted, protein expression levels were examined in a cell line using 293T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequencies of each of the HPA\u2010b genotypes were as follows: HPA\u20101b, 0.4%; HPA\u20102b, 11.8%; HPA\u20103b, 41.3%; HPA\u20104b, 0.8%; HPA\u20105b, 4.3%; HPA\u20106b, 1.9%; HPA\u201015b, 48.8%; HPA\u201021b, 0.6%; and \u201cb\u201d allotype in the other HPA systems, 0.0%. Twenty\u2010eight variants were found; nine of them were predicted to cause amino acid substitution. However, expression analysis revealed that they did not affect protein expression levels on the cell surface.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nine HPA systems are of primary importance in Japan in potentially triggering thrombocytopenia via the HPA antibodies. Similar studies in other countries or races, together with ours, could provide basic information for clinicians in multiethnic societies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15967","type":"journal-article","created":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T09:40:06Z","timestamp":1595670006000},"page":"2702-2713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Frequency of allotype \u201cb\u201d in human platelet antigen 1 to 29 systems among blood donors in Japan estimated using high\u2010resolution melt analysis"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1776-0052","authenticated-orcid":false,"given":"Tomoya","family":"Hayashi","sequence":"first","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryota","family":"Aminaka","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ishii","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Tani","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Osaka Blood Centre  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Takihara","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6518-2085","authenticated-orcid":false,"given":"Fumiya","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Japanese Red Cross Kinki Block Blood Centre  Ibaraki Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,25]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"380","article-title":"Advances in alloimmune thrombocytopenia: perspectives on current concepts of human platelet antigens, antibody detection strategies, and genotyping","volume":"13","author":"Hayashi T","year":"2015","journal-title":"Blood Transfus"},{"key":"e_1_2_8_3_1","unstructured":"EMBL\u2010EBI. All HPA Genetic Information.https:\/\/www.ebi.ac.uk\/ipd\/hpa\/table2.html. 2020. Accessed March 16 2020."},{"key":"e_1_2_8_4_1","unstructured":"Versiti. Human Platelet Antigen (HPA) Database. Available from:https:\/\/www.versiti.org\/hpa. c2020. Accessed April 20 2020."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36996420760.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1997.d01-72.x"},{"key":"e_1_2_8_7_1","first-page":"222","article-title":"Genomic restriction fragment length polymorphism typing of human platelet\u2010specific antigens in blood donors from southern Italy and in alloimmunised pregnancies","volume":"3","author":"Fratellanza G","year":"2005","journal-title":"Blood Transfus"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12507"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-7512-6_6"},{"issue":"3","key":"e_1_2_8_10_1","first-page":"285","article-title":"A preliminary evaluation of next\u2010generation sequencing as a screening tool for targeted genotyping of erythrocyte and platelet antigens in blood donors","volume":"16","author":"Orzi\u0144ska A","year":"2018","journal-title":"Blood Transfus"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000505161"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000504894"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01953.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03630.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2011.01981.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-011-0641-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01344.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03491.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02557.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2004.00537.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2004.00535.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02537.x"},{"issue":"4","key":"e_1_2_8_23_1","first-page":"549","article-title":"Increase in the number of patients with hematological disease receiving long\u2010term platelet transfusion at an outpatient clinic","volume":"49","author":"Sakai C","year":"2003","journal-title":"Jpn J Transfus Med"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/09537104.2016.1257785"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.15967","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15967","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.15967","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.15967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T17:54:06Z","timestamp":1693677246000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.15967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,25]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1111\/trf.15967"],"URL":"https:\/\/doi.org\/10.1111\/trf.15967","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2020,7,25]]},"assertion":[{"value":"2020-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}