{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:27:45Z","timestamp":1760956065910,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The study was funded by Johns Hopkins Hospital Transfusion Medicine Division: Dr. Bloch is supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health","award":["K23HL151826"],"award-info":[{"award-number":["K23HL151826"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In 2019, the United States Food and Drug Administration published its final recommendations to mitigate bacterial contamination of platelets. We sought to evaluate our secondary bacterial culture (SBC) strategy in light of those recommendations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A retrospective analysis was conducted of SBC data (October 2016\u20102019) at our institution. SBC was performed upon receipt (Day 3 after collection); 5 mL of platelet product was inoculated aseptically into an aerobic bottle and incubated at 35\u00b0C for 3\u2009days. For 8 months, a 10\u2010mL inoculum was trialed. No quarantine was applied. All positive cultures underwent Gram staining and repeat culture of the platelet product (if available). A probable true positive was defined as concordant positive culture between the initial and repeat culture. The incidence of probable true\u2010 and false\u2010positive cultures were reported descriptively and differences evaluated by sampling volume.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Over 3\u2009years, 55\u2009896 platelet products underwent SBC, yielding 30 initial positive results (approx. 1\/1863 platelets); 25 (83.3%) signaled within 24\u2009hours of SBC. The rates of probable true positive, false positive, and indeterminate for 5 mL were 0.027% (1\/3771), 0.002% (1\/45\u2009251) and 0.018% (1\/5656), respectively. The respective rates for 10 mL were 0.018% (1\/5323), 0.07% (1\/1521), and 0%. Seven of eight (87.5%) false\u2010positive SBCs occurred with a 10\u2010mL inoculum. No septic transfusion reactions were reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SBC continues to interdict bacterially contaminated units of platelets. Our findings suggest higher rates of false positivity using large\u2010volume inocula.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15978","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T20:55:51Z","timestamp":1596574551000},"page":"2021-2028","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Secondary bacterial culture of platelets to mitigate transfusion\u2010associated sepsis: A 3\u2010year analysis at a large academic institution"],"prefix":"10.1111","volume":"60","author":[{"given":"Alexander J.","family":"Fenwick","sequence":"first","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5354-3899","authenticated-orcid":false,"given":"Eric A.","family":"Gehrie","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christi E.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0517-3766","authenticated-orcid":false,"given":"Aaron A. 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R.","family":"Brunker","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland USA"},{"name":"American Red Cross Biomedical Services, Greater Chesapeake &amp; Potomac Region  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Shifflett","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology The Johns Hopkins University School of Medicine  Baltimore Maryland 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