{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:05:22Z","timestamp":1747188322188,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["17YF1417300"],"award-info":[{"award-number":["17YF1417300"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Le<jats:sup>y<\/jats:sup> antigen is a carbohydrate chain belonging to the ABH\u2010Lewis blood group family. Le<jats:sup>y<\/jats:sup> has been reported to be present on red blood cells (RBCs) and granulocytes, but its distribution and function in platelets remain unknown. There are a variety of glycoproteins on platelets, which may carry the Le<jats:sup>y<\/jats:sup> antigen. This study aims to investigate the expression pattern and the function of Le<jats:sup>y<\/jats:sup> in human platelets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design and methods<\/jats:title><jats:p>Flow cytometry, Western blot, and immunofluorescence assays were performed to determine Le<jats:sup>y<\/jats:sup> expression on human platelets. ADP (1.25\u201010 \u03bcM) and thrombin (0.05\u20101 IU\/mL) were used to activate platelets in the presence or absence of prostaglandin E1 (PGE1) and the Le<jats:sup>y<\/jats:sup> expression was evaluated again by flow cytometry. Blockade was performed with an anti\u2010Le<jats:sup>y<\/jats:sup> monoclonal antibody to verify the role of this epitope in platelet function. Finally, coimmunoprecipitation was performed to identify glycoproteins associated with Le<jats:sup>y<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Le<jats:sup>y<\/jats:sup> was expressed on human platelets independent of ABO blood type. Le<jats:sup>y<\/jats:sup> expression was decreased in a dose\u2010dependent manner after activation with either ADP or thrombin, and this effect could be partially reversed by PGE1. Anti\u2010 Le<jats:sup>y<\/jats:sup> mAb treatment increased alpha\u2010granule release and neutralized the inhibitory effect of the anti\u2010CD61 antibody on platelet aggregation. In addition, Le<jats:sup>y<\/jats:sup> was proven to interact and colocalize with CD61.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate nondifferential expression of Le<jats:sup>y<\/jats:sup> in platelets of different ABO blood types and suggest the involvement of Le<jats:sup>y<\/jats:sup> in platelet function, possibly via interaction with CD61.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.15981","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T13:18:59Z","timestamp":1596633539000},"page":"2130-2138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Blood group\u2010related antigen <scp>Le<sup>y<\/sup><\/scp> on human platelets and its involvement in platelet aggregation via a possible interaction with <scp>CD61<\/scp>"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-1476","authenticated-orcid":false,"given":"Huijun","family":"Zhu","sequence":"first","affiliation":[{"name":"Immunohematology Platelet Lab, Shanghai Blood Center  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Ma","sequence":"additional","affiliation":[{"name":"Immunohematology Platelet Lab, Shanghai Blood Center  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruishu","family":"Li","sequence":"additional","affiliation":[{"name":"Immunohematology Platelet Lab, Shanghai Blood Center  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Fu","sequence":"additional","affiliation":[{"name":"Immunohematology Platelet Lab, Shanghai Blood Center  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lu","sequence":"additional","affiliation":[{"name":"Immunohematology Platelet Lab, Shanghai Blood Center  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2016.110"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13328"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5301\/jbm.5000206"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2007.07.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000124298"},{"key":"e_1_2_8_7_1","first-page":"503","article-title":"Lewis(y) antigen (CD174) and apoptosis in gastric and colorectal carcinomas: correlations with clinical and prognostic parameters","volume":"21","author":"Baldus 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