{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:29:32Z","timestamp":1771547372567,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"am","delay-in-days":339,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006190","name":"Research and Development","doi-asserted-by":"publisher","award":["YF05\u201000128\u2010SN"],"award-info":[{"award-number":["YF05\u201000128\u2010SN"]}],"id":[{"id":"10.13039\/100006190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The ongoing outbreak of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) has caused great global concerns. In contrast to SARS, some SARS\u2010CoV\u20102\u2013infected people can be asymptomatic or have only mild nonspecific symptoms. Furthermore, there is evidence that SARS\u2010CoV\u20102 may be infectious during an asymptomatic incubation period. With the discovery that SARS\u2010CoV\u20102 can be detected in plasma or serum, blood safety is worthy of consideration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>We developed a nucleic acid test (NAT) screening system for SARS\u2010CoV\u20102 targeting nucleocapsid protein (N) and open reading frame 1ab (ORF 1ab) gene that could screen 5076 samples every 24\u2009hours. The 2019 novel coronavirus RNA standard was used to evaluate linearity of standard curves. Diagnostic sensitivity and reproducibility were evaluated using artificial SARS\u2010CoV\u20102. Specificity was evaluated with 61 other respiratory pathogens. Diagnostic performance was evaluated by testing two sputum and nine oropharyngeal swab specimens. The reverse transcription polymerase chain reaction (RT\u2010PCR) assay was used to screen SARS\u2010CoV\u20102 RNA in blood donor specimens collected during the outbreak of SARS\u2010CoV\u20102 in Chengdu.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Limits of detection of the SARS\u2010CoV\u20102 RT\u2010PCR assay for N and ORF 1ab gene were 12.5 and 27.58 copies\/mL, respectively. Intra\u2010assay and interassay for the SARS\u2010CoV\u20102 RT\u2010PCR assay based on cycle threshold were acceptably low. No cross\u2010reactivity was observed with other respiratory virus and bacterial isolates. The overall agreement value between the SARS\u2010CoV\u20102 RT\u2010PCR assay and clinical diagnostic results was 100%. A total of 16\u2009287 blood specimens collected from blood donors during SARS\u2010CoV\u20102 surveillance were tested negative.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A high\u2010throughput NAT screening system was developed for SARS\u2010CoV\u20102 screening of blood donations during the outbreak of SARS\u2010CoV\u20102.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16049","type":"journal-article","created":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T07:58:43Z","timestamp":1597478323000},"page":"2952-2961","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development and evaluation of a Novel <scp>RT\u2010PCR<\/scp> system for reliable and rapid <scp>SARS\u2010CoV<\/scp>\u20102 screening of blood donations"],"prefix":"10.1111","volume":"60","author":[{"given":"Meng","family":"Li","sequence":"first","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Chen","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongxia","family":"Luo","sequence":"additional","affiliation":[{"name":"Teaching &amp; Research Department Public Health and Clinical Center of Chengdu  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Luo","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Laboratory Public Health and Clinical Center of Chengdu  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jue","family":"Hou","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"Productresearch &amp; Development Department Sansure Biotechnology Company  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humin","family":"Liu","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufang","family":"Dong","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanjiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maosheng","family":"Ji","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caibing","family":"Wei","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3764-1787","authenticated-orcid":false,"given":"Hua","family":"Shan","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine  Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3371-6171","authenticated-orcid":false,"given":"Xuemei","family":"Fu","sequence":"additional","affiliation":[{"name":"Blood Research Laboratory Chengdu Blood Center  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2020.01.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2020.02.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17051679"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-020-00646-x"},{"key":"e_1_2_8_7_1","volume-title":"Statement on the second meeting of the International Health Regulations (2005) Emergency Committee regarding the outbreak of novel coronavirus (2019\u2010nCoV) [Internet]","author":"WHO","year":"2020"},{"key":"e_1_2_8_8_1","volume-title":"WHO director\u2010General's opening remarks at the media briefing on COVID\u201019 \u2010 march 11, 2020 [internet]","author":"WHO","year":"2020"},{"key":"e_1_2_8_9_1","volume-title":"Coronavirus disease (COVID\u20102019) situation reports \u2010 191 July 29, 2020 [internet]","author":"WHO","year":"2020"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa145"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_2_8_13_1","unstructured":"Y\u2010Z. Z. Novel2019 coronavirus genome.Virological. [internet].2020.http:\/\/virological.org\/t\/novel-2019-coronavirus-genome\/319. Accessed January 21 2020."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-020-00240-0"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1719902"},{"key":"e_1_2_8_16_1","article-title":"Features, evaluation and treatment Coronavirus (COVID\u201019) StatPearls","author":"Cascella M","year":"2020","journal-title":"Treasure Island (FL)"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25748"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2648"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.02.018"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30154-9"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa077"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30528-6"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2605.200198"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.03.027"},{"key":"e_1_2_8_26_1","volume-title":"Coronavirus disease 2019 (COVID\u201019) situation report \u221273 [internet]","author":"WHO","year":"2020"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-0504"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001316"},{"key":"e_1_2_8_29_1","first-page":"E021","article-title":"Analysis on the epidemic factors for the Corona virus disease","volume":"54","author":"Yang HY","year":"2020","journal-title":"Zhonghua Yu Fang Yi Xue Za Zhi"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.03.071"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1729071"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1732837"},{"key":"e_1_2_8_33_1","first-page":"1843","article-title":"Detection of SARS\u2010CoV\u20102 in different types of clinical specimens","volume":"323","author":"Wang W","year":"2020","journal-title":"JAMA"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2607.200839"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.04.004"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.12.2003261"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2020.03.009"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa345"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01884"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030747"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.10.2000180"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1661-4"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16049","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/trf.16049","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T01:23:19Z","timestamp":1693876999000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,10]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/trf.16049"],"URL":"https:\/\/doi.org\/10.1111\/trf.16049","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,10]]},"assertion":[{"value":"2020-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}