{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:18:34Z","timestamp":1790806714178,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>At Canadian Blood Services, platelet concentrate (PC) shelf life was extended to 7\u2009days with a large\u2010volume, delayed\u2010sampling bacterial screening algorithm. We present the development study and postimplementation results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>In the development study, PCs inoculated with five bacteria (various concentrations) were incubated for 7\u2009days with daily sampling for BacT\/ALERT cultures and bacterial quantification. After implementation, from August 2017 to December 2019, a total of 223\u2009156 pools and 39\u2009725 apheresis units and 5310 outdated PCs were screened. Since March 2018, cocomponents associated to false\u2010positive results have been released to inventory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the development study, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Serratia marcescens,<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> were detected at concentrations of at least 0.01\u2009colony\u2010forming units (CFUs)\/mL at 24\u2009hours postinoculation. However, <jats:italic>Staphylococcus epidermidis<\/jats:italic> was detected at concentrations of less than 0.16\u2009CFUs\/mL only more than 48\u2009hours postinoculation. After implementation, 776 (0.35%) and 303 (0.77%) initial\u2010positive results and 201 (0.09%) and 16 (0.04%) confirmed\u2010positive results were obtained for pools and apheresis units, respectively, predominantly with <jats:italic>Cutibacterium acnes<\/jats:italic>. Other organisms included staphylococci, streptococci, <jats:italic>Klebsiella oxytoca<\/jats:italic> and <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. One nonfatal reaction involving a 7\u2010day pool contaminated with <jats:italic>S. epidermidis<\/jats:italic> occurred<jats:italic>.<\/jats:italic> Approximately, 1\u2010in\u20101000 false\u2010negative screening results were obtained during testing of outdated PCs. Approximately 1000 cocomponents associated with false\u2010positive results were released into inventory. Combined PC outdating at Canadian Blood Services and hospitals was reduced from 18.9% to 13.1%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Screening of 7\u2010day PCs increased bacterial detection mainly of anaerobes and reduced outdating. The incidence of septic transfusion events has decreased approximately threefold. A longer surveillance period is needed to evaluate the value of anaerobic cultures and residual safety risk.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16112","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T03:41:51Z","timestamp":1604374911000},"page":"2918-2928","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Extension of platelet shelf life with an improved bacterial testing algorithm"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-4987","authenticated-orcid":false,"given":"Sandra","family":"Ramirez\u2010Arcos","sequence":"first","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"},{"name":"Department of Biochemistry, Microbiology and Immunology University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Evans","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri","family":"McIntyre","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Pang","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010Long","family":"Yi","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caesar","family":"DiFranco","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9904-9952","authenticated-orcid":false,"given":"Mindy","family":"Goldman","sequence":"additional","affiliation":[{"name":"Canadian Blood Services  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2212-9"},{"key":"e_1_2_9_3_1","first-page":"153","volume-title":"AABB transfusion reactions","author":"Ramirez\u2010Arcos S","year":"2012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499349"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2014.01.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14774"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000330312"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15733"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12561"},{"key":"e_1_2_9_10_1","unstructured":"U.S. Food and Drug Administration.Bacterial risk control strategies for blood collection establishments and transfusion services to enhance the safety and availability of platelets for transfusion guidance for industry.https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/bacterial\u2010risk\u2010control\u2010strategies\u2010blood\u2010collection\u2010establishments\u2010and\u2010transfusion\u2010services\u2010enhance. 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