{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T22:54:25Z","timestamp":1767826465595,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We previously reported a flow path\u2013ultraviolet C (UVC) irradiation system for platelet concentrates (PCs) with platelet additive solution (PAS) to minimize contamination by bacteria. Here, we investigated functionalities of irradiated platelets (PLTs) in in vitro thrombus formation and in vivo hemostasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>PAS\u2010PCs were irradiated with flash UVC using the flow path system. Their variables (PLT count, mean platelet volume, pH, glucose, lactate, glycoprotein [GP] Ib, and activated integrin \u03b1IIb\u03b23) were evaluated. Static adhesion to collagen or fibrinogen was analyzed using fluorescent microscopy. Thrombus formation under flow conditions was assessed using a collagen\u2010coated bead column. Adenosine diphosphate (ADP)\u2010induced Akt phosphorylation was determined by western blot. In vivo hemostasis and circulatory survival of PLTs were assessed with a rabbit bleeding model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All variables, except for GPIb expression, were slightly, but significantly, impaired after flash UVC irradiation throughout the 6\u2010day storage period. No difference was observed in static adhesion to either collagen or fibrinogen between irradiated and nonirradiated PAS\u2010PCs. In vitro thrombus formation of flash UVC\u2010irradiated PAS\u2010PCs was significantly greater than that of nonirradiated PAS\u2010PCs. ADP\u2010induced Akt phosphorylation was enhanced in irradiated PAS\u2010PCs. In vivo hemostatic efficacy was comparable between the groups on Day 1. The efficacy declined in nonirradiated PAS\u2010PCs on Day 5, while it was retained in flash UVC\u2010irradiated PAS\u2010PCs. Circulatory survival of PLTs was lower in irradiated PAS\u2010PCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PAS\u2010PCs irradiated with UVC from xenon flash have favorable properties to achieve hemostasis compared with nonirradiated PAS\u2010PCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16138","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T09:48:44Z","timestamp":1603792124000},"page":"191-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In vitro thrombus formation and in vivo hemostasis mediated by platelets irradiated with bactericidal ultraviolet <scp>C<\/scp> from xenon flash under flow conditions"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0881-0089","authenticated-orcid":false,"given":"Hideki","family":"Abe","sequence":"first","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimika","family":"Endo","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Nogawa","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"},{"name":"Division of Hematology, Department of Medicine Keio University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-1955","authenticated-orcid":false,"given":"Masayuki","family":"Shiba","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Miyata","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4660-6839","authenticated-orcid":false,"given":"Masahiro","family":"Satake","sequence":"additional","affiliation":[{"name":"Central Blood Institute, Blood Service Headquarters Japanese Red Cross Society  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1991.03470040119033"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.15594"},{"key":"e_1_2_8_4_1","unstructured":"Japanese Red Cross Society Blood Service Headquarters. Transfusion\u2010transmitted bacterial infection [monograph on the Internet]. 2018. [cited Jan 29]. 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