{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:19:12Z","timestamp":1788347952295,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Epstein\u2010Barr virus (EBV) is carried in the blood of most adults, and transfusion\u2010related infections have been reported. EBV is particularly deleterious in immunosuppressed transplant patients. The aim was to determine if EBV transmission occurred through leukodepleted blood product transfusion in pediatric recipients of hematopoietic stem cell transplants (HSCT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>This prospective Canadian multi\u2010center cohort study includes 156 allogeneic HSCT pediatric recipients. The association between EBV and transfusion was analyzed using Cox regressions. EBV infection, defined by a PCR+ test in the blood of seronegative recipients of an EBV\u2010negative graft, was monitored in order to correlate the recipient EBV strain with that of the blood donors. EBV genotypes were determined by PCR amplification followed by DNA sequencing at two loci (EBNA3b and LMP1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No statistically significant associations were found between transfusions and EBV. One case of post\u2010transplant EBV infection was identified among the 21 EBV\u2010seronegative recipients receiving an EBV\u2010negative graft. A total of 22 blood donors were retraced to determine whether the recipient\u02bcs EBV strain matched that of a donor. One donor strain showed 100% sequence homology at the EBNA3b locus, but differed by one or two point mutations and by a 132\u2010bp deletion at the LMP1 locus. The blood donor in question was alone among the 22 donors to show amplifiable virus in plasma. Blood from this donor readily produced an immortalized lymphoblastoid cell line in culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While considered a rare event, EBV transmission through transfusion may occur in the context of severe immunosuppression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16149","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T15:11:02Z","timestamp":1603379462000},"page":"144-158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Transfusion\u2010related <scp>Epstein\u2010Barr<\/scp> virus (<scp>EBV<\/scp>) infection: A multicenter prospective cohort study among pediatric recipients of hematopoietic stem cell transplants (<scp>TREASuRE<\/scp> study)"],"prefix":"10.1111","volume":"61","author":[{"given":"Pascal R.","family":"Enok Bonong","sequence":"first","affiliation":[{"name":"Department of Social and Preventive Medicine Universit\u00e9 de Montr\u00e9al, Sainte\u2010Justine University Hospital  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Buteau","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics Sainte\u2010Justine University Hospital, Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Delage","sequence":"additional","affiliation":[{"name":"Medical Affairs, Microbiology H\u00e9ma\u2010Qu\u00e9bec  Ville St\u2010Laurent Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome E.","family":"Tanner","sequence":"additional","affiliation":[{"name":"Sainte\u2010Justine University Hospital  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Lacroix","sequence":"additional","affiliation":[{"name":"Division of Pediatric Intensive Care Medicine, Department of Pediatrics Sainte\u2010Justine University Hospital, Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Duval","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Pediatrics Sainte\u2010Justine University Hospital, Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Laporte","sequence":"additional","affiliation":[{"name":"Sainte\u2010Justine University Hospital  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Tucci","sequence":"additional","affiliation":[{"name":"Division of Pediatric Intensive Care Medicine, Department of Pediatrics Sainte\u2010Justine University Hospital, Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Robitaille","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Pediatrics Sainte\u2010Justine University Hospital, Universit\u00e9 de Montr\u00e9al and Medical Affairs, Transfusion Medicine, H\u00e9ma\u2010Qu\u00e9bec  Ville Saint\u2010Laurent Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1721-0541","authenticated-orcid":false,"given":"Philip C.","family":"Spinella","sequence":"additional","affiliation":[{"name":"St. Louis Children\u02bcs Hospital Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Cuvelier","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Child Health CancerCare Manitoba, University of Manitoba  Winnipeg Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Vercauteren","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine BC Children\u02bcs Hospital, University of British Colombia  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Department of Oncology Alberta Children\u02bcs Hospital, University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Fearon","sequence":"additional","affiliation":[{"name":"Medical Microbiology Canadian Blood Services  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-1109","authenticated-orcid":false,"given":"Steven J","family":"Drews","sequence":"additional","affiliation":[{"name":"Medical Affairs and Innovation, Microbiology Canadian Blood Services and University of Alberta, Laboratory Medicine and Pathology  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Infectiology and Immunology Universit\u00e9 de Montr\u00e9al, Sainte\u2010Justine University Hospital Research Centre, Universit\u00e9 de Montr\u00e9al  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-7837","authenticated-orcid":false,"given":"Helen","family":"Trottier","sequence":"additional","affiliation":[{"name":"Department of Social and Preventive Medicine Universit\u00e9 de Montr\u00e9al, Sainte\u2010Justine University Hospital  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/522531"},{"issue":"2","key":"e_1_2_8_3_1","first-page":"89","article-title":"Epstein\u2010Barr virus infection in transplant recipients: Summary of a workshop on surveillance, prevention and treatment","volume":"13","author":"Allen U","year":"2002","journal-title":"Can J Infect Dis"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.150"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000265914.16491.7d"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199103000-00005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.5.876"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.9.2715.2715"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.2.976-980.1991"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-178046"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2013.84"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31829d38af"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.087338"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13150"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.144428"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2019.02.016"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2013.190"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.07.035"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.972"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05287.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2010.00564.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.03.025"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10751"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23452"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.26"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-019-03742-7"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05439.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/petr.12620"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.221"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703331"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2009.00410.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2016.12.635"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705703"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12172"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13095"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.2.812.bloodjournal872812"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01685.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365540260348626"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03611.x"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1086\/379834"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-144-37586"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2004.09.005"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03614-14"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20151"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1214\/aos\/1176350951"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113677"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwn164"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/1536867X0400400403"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001648-200009000-00012"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwu125"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001648-200009000-00011"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v043.i13"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-006-9008-y"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2014.0037"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ede.0000135174.63482.43"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/0962280216628900"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.5984"},{"key":"e_1_2_8_58_1","volume-title":"An introduction to survival analysis using Stata","author":"Cleves MA","year":"2016"},{"key":"e_1_2_8_59_1","volume-title":"Canadian Blood Services","author":"Sher GD","year":"1999"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2005.04303.x"},{"key":"e_1_2_8_61_1","first-page":"138","article-title":"Relevance of transmission of Epstein\u2010Barr virus through blood transfusion","volume":"32","author":"Wagner HJ","year":"1994","journal-title":"Beitr Infusionsther Transfusionsmed"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02197.x"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12330"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3740"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00782"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198405103101905"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1086\/491740"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.9908-9916.1999"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16149","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16149","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T11:56:40Z","timestamp":1693655800000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,22]]},"references-count":67,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1111\/trf.16149"],"URL":"https:\/\/doi.org\/10.1111\/trf.16149","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,22]]},"assertion":[{"value":"2020-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}