{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T13:56:18Z","timestamp":1752674178611,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T00:00:00Z","timestamp":1604880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Therapeutic phlebotomy is the standard treatment of hereditary hemochromatosis (HH), the most common genetic disease in people of Northern European descent. Red cell concentrates from HH donors have been reported safe for transfusion, but little data is available on the storage properties of platelet concentrates from HH donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Whole blood was collected from 10 healthy individuals and 10 newly diagnosed HH patients with elevated serum ferritin. Platelet\u2010rich plasma (PRP) was prepared and split into four 20\u2010mL\u2009units. Platelet quality tests were performed on days 0, 1, 3, 5, and 7 of storage, including platelet aggregation (ADP, arachidonic acid, collagen, and epinephrine agonists), blood gas analysis, flow cytometry (CD41, CD42b, and CD62P expression), and ELISA (sCD40L and sCD62p in supernatant).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean serum ferritin levels were higher in HH patients than in controls (847.5 vs 45.8\u2009ng\/mL, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). Overall, no difference in quality test results was observed between the two study groups over 7\u2010day storage (<jats:italic>P<\/jats:italic>\u2009&gt;\u2009.05), including blood gas analysis, platelet aggregation, and expression of surface (CD62p and CD42b) and secreted (sCD62P and sCD40L) activation markers. Expected alterations in metabolic (CO<jats:sub>2<\/jats:sub> and glucose decrease, O<jats:sub>2<\/jats:sub> and lactate increase, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) and platelet activation markers (CD42b decrease, CD62P increase, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05) over time were observed in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although these findings indicate that platelets of individuals with HH are comparable to platelets from healthy donors, more extensive studies are needed before definite conclusions can be drawn.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16176","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:05:39Z","timestamp":1604966739000},"page":"202-211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A comparison of platelet quality between platelets from healthy donors and hereditary hemochromatosis donors over seven\u2010day storage"],"prefix":"10.1111","volume":"61","author":[{"given":"Marta","family":"Mikaelsdottir","sequence":"first","affiliation":[{"name":"Faculty of Medicine University of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brynjar","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Department of Hematology Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudmundur","family":"Runarsson","sequence":"additional","affiliation":[{"name":"Department of Hematology Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Una","family":"Bjarnadottir","sequence":"additional","affiliation":[{"name":"Department of Immunology Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pall T.","family":"Onundarson","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Iceland  Reykjavik Iceland"},{"name":"Department of Hematology Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4968-842X","authenticated-orcid":false,"given":"Olafur E.","family":"Sigurjonsson","sequence":"additional","affiliation":[{"name":"The Blood Bank Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"},{"name":"School of Science and Engineering Reykjavik University  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3800-1223","authenticated-orcid":false,"given":"Anna M.","family":"Halldorsdottir","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Iceland  Reykjavik Iceland"},{"name":"The Blood Bank Landspitali \u2013 The National University Hospital of Iceland  Reykjavik Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1038\/nrdp.2018.16"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.3109\/09537104.2011.634933"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1080\/17474124.2018.1496016"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1002\/hep.29521"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1016\/j.jhep.2012.04.040"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1111\/vox.12038"},{"issue":"48","key":"e_1_2_7_8_1","first-page":"809","article-title":"Platelet transfusion in hematology, oncology and surgery","volume":"111","author":"Wandt H","year":"2014","journal-title":"Dtsch Arztebl Int."},{"issue":"1","key":"e_1_2_7_9_1","first-page":"5","article-title":"Platelet transfusions: trigger, dose, benefits, and risks","volume":"2","author":"Blumberg N","year":"2010","journal-title":"F1000 Med Rep."},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1097\/MOH.0000000000000188"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1182\/asheducation-2006.1.492"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.4103\/0973-6247.150933"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1034\/j.1600-0609.2003.00029.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1007\/s10517-010-0814-6"},{"doi-asserted-by":"publisher","key":"e_1_2_7_15_1","DOI":"10.1136\/bmj.320.7245.1314"},{"volume-title":"Platelets","year":"2013","author":"Michelson AD","key":"e_1_2_7_16_1"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_1","DOI":"10.1111\/trf.13890"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_1","DOI":"10.1016\/j.cll.2010.02.002"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_1","DOI":"10.1182\/blood.V46.2.209.209"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_1","DOI":"10.1016\/j.transci.2009.07.002"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_1","DOI":"10.1182\/blood.V52.2.425.425"},{"doi-asserted-by":"publisher","key":"e_1_2_7_22_1","DOI":"10.1046\/j.1365-3148.2000.00243.x"},{"key":"e_1_2_7_23_1","first-page":"243","article-title":"Evaluation of platelet storage lesions in platelet concentrates stored for seven days","volume":"118","author":"Singh H","year":"2003","journal-title":"Indian J Med Res."},{"doi-asserted-by":"publisher","key":"e_1_2_7_24_1","DOI":"10.1016\/j.transci.2011.03.008"},{"issue":"2","key":"e_1_2_7_25_1","first-page":"182","article-title":"Storage of buffy\u2010coat\u2010derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit","volume":"9","author":"Sandgren P","year":"2011","journal-title":"Blood Transfus."},{"doi-asserted-by":"publisher","key":"e_1_2_7_26_1","DOI":"10.1111\/j.1423-0410.2005.00609.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_27_1","DOI":"10.1111\/j.1423-0410.2008.01042.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_28_1","DOI":"10.1111\/j.1423-0410.2006.00771.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_29_1","DOI":"10.1046\/j.1537-2995.1997.37597293876.x"},{"issue":"4","key":"e_1_2_7_30_1","first-page":"480","article-title":"Evaluation of platelet function during extended storage in additive solution, prepared in a new container that allows manual buffy\u2010coat platelet pooling and leucoreduction in the same system","volume":"10","author":"Plaza EM","year":"2012","journal-title":"Blood Transfus."},{"doi-asserted-by":"publisher","key":"e_1_2_7_31_1","DOI":"10.5581\/1516-8484.20110030"},{"doi-asserted-by":"publisher","key":"e_1_2_7_32_1","DOI":"10.1111\/j.1365-2141.1988.tb04179.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_33_1","DOI":"10.1016\/S0049-3848(02)00338-9"},{"doi-asserted-by":"publisher","key":"e_1_2_7_34_1","DOI":"10.1097\/MOH.0b013e3283497dfb"},{"doi-asserted-by":"publisher","key":"e_1_2_7_35_1","DOI":"10.1080\/09537100802302225"},{"doi-asserted-by":"publisher","key":"e_1_2_7_36_1","DOI":"10.1111\/trf.13026"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16176","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16176","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16176","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T11:56:19Z","timestamp":1693655779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,9]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1111\/trf.16176"],"URL":"https:\/\/doi.org\/10.1111\/trf.16176","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"type":"print","value":"0041-1132"},{"type":"electronic","value":"1537-2995"}],"subject":[],"published":{"date-parts":[[2020,11,9]]},"assertion":[{"value":"2020-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}