{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:00:50Z","timestamp":1791900050589,"version":"4.3.4"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>As a method with insignificant adverse effects on in vitro quality of platelet concentrates (PCs), gamma irradiation is applied to abrogate the risk of transfusion\u2010associated graft\u2010vs\u2010host disease in vulnerable recipients. However, there is some evidence of lower posttransfusion responses and proteomic alterations in gamma\u2010irradiated platelets (PLTs), which raises some questions about their quality, safety, and efficacy. Since reactive oxygen species (ROS) are considered as markers of PLT storage lesion (PSL), the study presented here investigated oxidant state in gamma\u2010irradiated PCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      PLT\u2010rich plasma PC was split into two bags, one kept as control while other was subjected to gamma irradiation. Within 7\u2009days of storage, the levels of intra\u2010PLT superoxide, H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      , mitochondrial ROS, P\u2010selectin expression, and phosphatidylserine (PS) exposure were detected by flow cytometry while intracellular reduced glutathione (GSH), glucose concentration, and lactate dehydrogenase (LDH) activity were measured by enzymocolorimetric method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GSH decreased, while ROS generation and LDH activity increased, during storage. Gamma irradiation significantly attenuated GSH whereas increased ROS generation in earlier and later stages of storage associated with either P\u2010selectin or PS exposure increments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Gamma irradiation can significantly increase cytosolic ROS generation in two distinct phases, one upon irradiation and another later in longer\u2010stored PCs. While earlier ROS influx seems to be governed by direct effect of irradiation, the second phase of oxidant stress is presumably due to the storage\u2010dependent PLT activation. Intriguingly, these observations were also in line with early P\u2010selectin increments and increased PS exposure in longer\u2010stored PLTs. Given the mutual link between ROS generation and PLT activation, further investigation is required to explore the effect of gamma irradiation on the induction of PSL.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.16207","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T16:50:38Z","timestamp":1606236638000},"page":"579-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The effect of gamma irradiation on platelet redox state during storage"],"prefix":"10.1111","volume":"61","author":[{"given":"Fatemeh Kiani","family":"Nodeh","sequence":"first","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehteramolsadat","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1128-5649","authenticated-orcid":false,"given":"Mehran","family":"Ghasemzadeh","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.04.057"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2016.1171303"},{"key":"e_1_2_10_4_1","first-page":"713","article-title":"Insights into platelet storage and the need for multiple approaches","volume":"45","author":"Handigund M","year":"2015","journal-title":"Ann Clin Lab 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