{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:49:56Z","timestamp":1790696996481,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T00:00:00Z","timestamp":1613174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patients can form antibodies to foreign human leukocyte antigen (HLA) Class I antigens after exposure to allogeneic cells. These anti\u2010HLA class I antibodies can bind transfused platelets (PLTs) and mediate their destruction, thus leading to PLT refractoriness. Patients with PLT refractoriness need HLA\u2010matched PLTs, which require expensive HLA typing of donors, antibody analyses of patient sera and\/or crossmatching. An alternative approach is to reduce PLT HLA Class I expression using a brief incubation in citric acid on ice at low pH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Materials<\/jats:title><jats:p>Apheresis PLT concentrates were depleted of HLA Class I complexes by 5 minutes incubation in ice\u2010cold citric acid, at pH 3.0. Surface expression of HLA Class I complexes, CD62P, CD63, phosphatidylserine, and complement factor C3c was analyzed by flow cytometry. PLT functionality was tested by thromboelastography (TEG).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acid treatment reduced the expression of HLA Class I complexes by 71% and potential for C3c binding by 11.5\u2010fold compared to untreated PLTs. Acid\u2010treated PLTs were significantly more activated than untreated PLTs, but irrespective of this increase in steady\u2010state activation, CD62P and CD63 were strongly upregulated on both acid\u2010treated and untreated PLTs after stimulation with thrombin receptor agonist peptide. Acid treatment did not induce apoptosis over time. X\u2010ray irradiation did not significantly influence the expression of HLA Class I complexes, CD62P, CD63, and TEG variables on acid treated PLTs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The relatively simple acid stripping method can be used with irradiated apheresis PLTs and may prevent transfusion\u2010associated HLA sensitization and overcome PLT refractoriness.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16282","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T17:27:57Z","timestamp":1613323677000},"page":"1222-1234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp>HLA<\/scp> class <scp>I<\/scp> depletion by citric acid, and irradiation of apheresis platelets for transfusion of refractory patients"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0562-0025","authenticated-orcid":false,"given":"Mohammad Reza","family":"Mirlashari","sequence":"first","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Vetlesen","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise Sofie H.","family":"Nissen\u2010Meyer","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Naper","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Oslo University 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