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Little empirical evidence exists regarding the determination of donor eligibility and experiences with donor selection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>This prospective study was conducted at a tertiary care hospital in New York to select plasma donors for a randomized, double\u2010blind, controlled convalescent plasma trial. Clearance for donation required successful completion of an online questionnaire and an in\u2010person screening visit, which included (a) completion of a Donor Health Questionnaire (DHQ), (b) Immunoglobulin G (IgG) antibody testing using an immunochromatographic anti\u2010 severe acute respiratory coronavirus 2 (SARS\u2010CoV\u20102) test, (c) Polymerase chain reaction (PCR) testing if &lt;28\u2009days from symptom resolution, and (d) routine blood bank testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After receiving 3093 online questionnaires, 521 individuals presented for in\u2010person screening visits, with 40.1% (n = 209) fully qualifying. Subjects (n = 312) failed to progress due to the following reasons: disqualifying answer from DHQ (n = 30, 9.6%), insufficient antibodies (n = 198, 63.5%), persistent positive PCR tests (n = 14, 4.5%), and blood donation testing labs (n = 70, 22.4%). Importantly, 24.6% and 11.1% of potential donors who reported having PCR\u2010diagnosed infection had low or undetectable SARS\u2010CoV\u20102 antibody levels, respectively. Surprisingly, 62.9% (56\/89) of subjects had positive PCR tests 14\u201327\u2009days after symptom resolution, with 13 individuals continuing to be PCR positive after 27\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is feasible for a single site to fully qualify a large number of convalescent plasma donors in a short period of time. Among otherwise qualified convalescent plasma donors, we found high rates of low or undetectable antibody levels and many individuals with persistently positive PCR tests.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16293","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T10:33:30Z","timestamp":1612953210000},"page":"1461-1470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of serological and <scp>PCR<\/scp> testing requirements on the selection of <scp>COVID<\/scp>\u201019 convalescent plasma donors"],"prefix":"10.1111","volume":"61","author":[{"given":"Jason A.","family":"Carter","sequence":"first","affiliation":[{"name":"MSTP Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex T.","family":"Freedenberg","sequence":"additional","affiliation":[{"name":"Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamie L.","family":"Romeiser","sequence":"additional","affiliation":[{"name":"Biostatistics, Department of Anesthesiology Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lillian R.","family":"Talbot","sequence":"additional","affiliation":[{"name":"MSTP Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Browne","sequence":"additional","affiliation":[{"name":"Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan E.","family":"Cosgrove","sequence":"additional","affiliation":[{"name":"Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret E.","family":"Shevik","sequence":"additional","affiliation":[{"name":"MSTP Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura M.","family":"Generale","sequence":"additional","affiliation":[{"name":"Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly G.","family":"Rago","sequence":"additional","affiliation":[{"name":"Cancer Center Clinical Trials Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppina A.","family":"Caravella","sequence":"additional","affiliation":[{"name":"Cancer Center Clinical Trials Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahmeena","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pathology\/Blood Bank Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda J.","family":"Mamone","sequence":"additional","affiliation":[{"name":"Department of Pathology\/Blood Bank Renaissance School of Medicine at Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8659-8098","authenticated-orcid":false,"given":"Elliott","family":"Bennett\u2010Guerrero","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Stony Brook University  Stony Brook New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"The Stony Brook Medicine COVID Plasma Trial Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30120-1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.20153"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2002032"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu396"},{"key":"e_1_2_10_6_1","first-page":"152","article-title":"Convalescent plasma: New evidence for an old therapeutic tool?","volume":"14","author":"Marano G","year":"2015","journal-title":"Blood Transfus"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2004168117"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","unstructured":"Jin C. 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