{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:56:47Z","timestamp":1790089007163,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We hypothesized that variability in practice exists for newborn immunohematology testing due to lack of consensus guidelines. We report the results of a survey assessing that variability at hospitals in the United States and Canada.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>An AABB Pediatric Subsection working party developed and validated a survey of newborn immunohematology testing practice. The survey was sent electronically to transfusion service leadership at teaching institutions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The response rate was 67% (61\/91); 56 surveys meeting inclusion criteria were analyzed. Approximately 90% (50\/56) were from birth hospitals and 16.1% (9\/56) were from pediatric hospitals. Newborn immunohematology testing is ordered as a panel by 66.0% (33\/50) of birth hospitals. ABO group and DAT is mandated before discharge in 14\/56 (25.0%) and 13\/56 (23.2%), respectively. About 76.8% (43\/56) selectively perform a DAT according to blood blank or clinical parameters. The most common DAT practices include anti\u2010IgG only testing by 73.2% (41\/56) and use of umbilical cord specimen type by 67.9% (38\/56). A positive DAT is a critical value for 26.8% (15\/56) and followed with eluate testing when a maternal antibody screen is positive for 48.2% (27\/56). In the setting of a non\u2010ABO maternal red cell antibody, 55.4% (31\/56), phenotype neonatal red cells when the DAT is positive. Group O RBC are transfused irrespective of the DAT result for 82.1%, (46\/56).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There is variability in newborn immunohematology testing and transfusion practice and potential overutilization of the DAT. Evidence\u2010based consensus guidelines should be developed to standardize practice and to improve safety.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16335","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T10:09:20Z","timestamp":1614247760000},"page":"1080-1092","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Survey of newborn direct antiglobulin testing practice in <scp>United States<\/scp> and <scp>Canadian<\/scp> transfusion services"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1910-2800","authenticated-orcid":false,"given":"Elizabeth P.","family":"Crowe","sequence":"first","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine New York Presbyterian Hospital, Weill Cornell Medicine  New York New York USA"},{"name":"Division of Transfusion Medicine, Department of Pathology Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9653-9905","authenticated-orcid":false,"given":"Ruchika","family":"Goel","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology Johns Hopkins University  Baltimore Maryland USA"},{"name":"Division of Hematology\/Oncology Simmons Cancer Institute at Southern Illinois University School of Medicine  Springfield Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Department of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8736-221X","authenticated-orcid":false,"given":"Erin K.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trisha E.","family":"Wong","sequence":"additional","affiliation":[{"name":"Pathology and Pediatric Hematology Oncology Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven R.","family":"Sloan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan","family":"Delaney","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine Division Children's National Hospital  Washington District of Columbia USA"},{"name":"Department of Pathology &amp; Pediatrics The George Washington University Medical School  Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3633-0728","authenticated-orcid":false,"given":"Lani","family":"Lieberman","sequence":"additional","affiliation":[{"name":"University Health Network and Affiliated Hospitals  Toronto Ontario Canada"},{"name":"Department of Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8042-1494","authenticated-orcid":false,"given":"Melissa M.","family":"Cushing","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine New York Presbyterian Hospital, Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01625.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2018.07.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23218"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0444-RS"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12299"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41111445.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-0329"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.114.1.297"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-1919"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedneo.2014.11.005"},{"key":"e_1_2_7_12_1","unstructured":"National Collaborating Centre for Women's and Children's Health (UK) Neonatal Jaundice"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2007.00767.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2012.26"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2013.6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1754.2005.00692.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedneo.2015.11.006"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2013-305553"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqx159"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16335","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16335","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T12:36:57Z","timestamp":1693571817000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,25]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1111\/trf.16335"],"URL":"https:\/\/doi.org\/10.1111\/trf.16335","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,25]]},"assertion":[{"value":"2020-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}