{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:26:43Z","timestamp":1781796403195,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,13]],"date-time":"2021-03-13T00:00:00Z","timestamp":1615593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oxaliplatin, a third\u2010generation platinum derivative is commonly used in combination treatment of metastatic colorectal cancer. Since 2008, it is the second most common cause of drug\u2010induced immune hemolytic anemia (DIIHA) investigated in our laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Samples from fifteen patients including nine (60%) with intravascular hemolysis, suspected of having DIIHA were studied for the presence of anti\u2010oxaliplatin. Direct antiglobulin tests (DATs) and tests with oxaliplatin\u2010treated red blood cells (RBCs) or untreated and enzyme\u2010treated RBCs in the presence of oxaliplatin were performed. A pool of normal AB sera with no unexpected antibodies was used as a control for nonimmunologic protein adsorption (NIPA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eleven (73%) of the fifteen patients had antibodies to oxaliplatin that reacted with drug\u2010treated RBCs and untreated RBCs in the presence of drug by tube and\/or gel method. Lower\u2010titer reactivity (&lt;20) obtained with four patients' sera and the corresponding pooled normal sera was most likely due to NIPA. Eighty seven percent (13\/15) of the patients had positive DAT either with anti\u2010IgG only (33%), IgG\u2009+\u2009C3d (40%), or C3d only (13%). Two patients had a negative DAT. No directly agglutinating antibody was observed with the pools of normal donor's sera in the presence of oxaliplatin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010oxaliplatin can cause severe intravascular hemolysis. Complement can usually be detected on the patient's RBCs and anti\u2010oxaliplatin can be detected in the patient's serum. RBC\u2010bound albumin detection with anti\u2010human albumin needs to be performed to confirm NIPA which could have contributed to the patient's hemolytic anemia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16360","type":"journal-article","created":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T13:10:52Z","timestamp":1615295452000},"page":"1609-1616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Serologic characteristics of oxaliplatin antibodies in 15 patients with drug\u2010induced immune hemolytic anemia"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2630-4980","authenticated-orcid":false,"given":"Tan Ngoc","family":"Nguyen","sequence":"first","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang, Laboratoire de Biologie M\u00e9dicale, Site Saint\u2010Antoine  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Maenulein","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang, Laboratoire de Biologie M\u00e9dicale, Site Saint\u2010Antoine  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentine","family":"Fihman","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang, Laboratoire de Biologie M\u00e9dicale  Site Ivry, 122\u2010130 rue Marcel Hartmann 94200 Ivry\u2010sur\u2010Seine France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Vinatier","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang, Laboratoire de Biologie M\u00e9dicale, Site Saint\u2010Antoine  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Moh Klaren","sequence":"additional","affiliation":[{"name":"Etablissement Fran\u00e7ais du Sang, Laboratoire de Biologie M\u00e9dicale, Site Saint\u2010Antoine  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2000.18.16.2938"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032709"},{"key":"e_1_2_7_4_1","first-page":"30259","article-title":"Acute oxaliplatin\u2010induced hemolytic anemia, thrombocytopenia and renal failure: Case report and literature review","volume":"1533","author":"Phull P","year":"2016","journal-title":"Clin Colorectal Cancer"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)03827-1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.02028.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12469"},{"key":"e_1_2_7_8_1","first-page":"415","article-title":"Mechanism of nonimmunologic adsorption of proteins using cephalothin\u2010coated red cells","volume":"24","author":"Branch DR","year":"1985","journal-title":"Transfusion"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03040.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tracli.2020.06.003"},{"key":"e_1_2_7_11_1","first-page":"700","volume-title":"Technical manual","author":"Brecher ME","year":"2002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2019.11.001"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01173.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.03111.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-7563.2005.00001.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00562.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1985.tb04069.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(90)70249-0"},{"key":"e_1_2_7_19_1","first-page":"33","volume-title":"Clinical and basic science aspects of immunohematology","author":"Garratty G","year":"1991"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16360","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.16360","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.16360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T07:08:09Z","timestamp":1693552089000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.16360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,13]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1111\/trf.16360"],"URL":"https:\/\/doi.org\/10.1111\/trf.16360","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,13]]},"assertion":[{"value":"2020-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}