{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:20:05Z","timestamp":1768512005354,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T00:00:00Z","timestamp":1626393600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There is a global increase in whole blood usage and at the same time, emerging pathogens give cause for pathogen reduction technology (PRT). The Mirasol PRT has shown promising results for plasma and platelet concentrate products. Treatment of whole blood with subsequent platelet survival and recovery analysis would be of global value.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A two\u2010arm, open\u2010label laboratory study was performed with 40 whole blood collections in four groups: non\u2010leukoreduced non\u2010PRT\u2010treated, non\u2010leukoreduced PRT\u2010treated, leukoreduced non\u2010PRT\u2010treated, and leukoreduced PRT\u2010treated.<\/jats:p><jats:p>Leukoreduction and\/or PRT\u2010treatment was performed on the day of collection, then all WB units were stored at room temperature for 24\u2009h. Sampling was performed after hold\u2010time and after 24\u2010h storage in RT. If PRT\u2010treatment or leukoreduction, samples were also taken subsequently after treatment.<\/jats:p><jats:p>Thirteen healthy volunteer blood donors completed the in vivo study per protocol. All WB units were non\u2010leukoreduced and PRT\u2010treated. Radioactive labeling of platelets from RT\u2010stored, PRT\u2010treated whole blood, sampling of subjects, recovery, and survival calculations were performed according to the Biomedical Excellence for Safer Transfusion Collaborative protocol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro characteristics show that PRT\u2010treatment leads to increased levels of hemolysis, potassium, and lactate, while there are decreased levels of glucose, FVIII, and fibrinogen after 24\u2009h of storage. All values are within requirements for WB.<\/jats:p><jats:p>In vivo recovery and survival of platelets were 85.4% and 81.3% of untreated fresh control, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PRT\u2010treatment moderately reduces whole blood quality but is well within the limits of international guidelines. Recovery and survival of platelets are satisfactory after Mirasol treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.16500","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T13:13:28Z","timestamp":1626441208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vitro characteristics and in vivo platelet quality of whole blood treated with riboflavin and <scp>UVA<\/scp>\/<scp>UVB<\/scp> light and stored for 24 hours at room temperature"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6798-1630","authenticated-orcid":false,"given":"Turid Helen Felli","family":"Lunde","sequence":"first","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Haukeland University Hospital  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsay","family":"Hartson","sequence":"additional","affiliation":[{"name":"Terumo BCT  Lakewood Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn Lawrence","family":"Bailey","sequence":"additional","affiliation":[{"name":"Platelet Transfusion Research Laboratory Bloodworks Northwest Research Institute  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9441-253X","authenticated-orcid":false,"given":"Tor Audun","family":"Hervig","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Haukeland University Hospital  Bergen Norway"},{"name":"Department of Clinical Science University of Bergen  Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12329"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12044"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2016.08.018"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e3182755dcc"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/voxs.12070"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.11604\/pamj.2015.20.119.5263"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13819"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12375"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1992.32292180149.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199312000-00034"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(97)80006-5"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1118\/1.4967482"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2009.10.016"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03715.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2003.00607.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.01020.x"},{"key":"e_1_2_7_18_1","volume-title":"Technical manual","author":"Fung MK","year":"2016"},{"key":"e_1_2_7_19_1","volume-title":"Guide to the preparation, use, and quality assurance of blood components","author":"Europe, C.o","year":"2017"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12364"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12047"},{"key":"e_1_2_7_22_1","first-page":"186","article-title":"The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution","volume":"8","author":"Reikvam H","year":"2010","journal-title":"Blood Transfus"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12895"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13959"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02714.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000287318.94088.d7"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12133"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13895"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13579"},{"key":"e_1_2_7_30_1","volume-title":"Guidance for industry and FDA review staff: collection of platelets by automated methods","author":"U.D.o.H.a.H.S. 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